1pwo: Difference between revisions

Line 1:

[[Image:1pwo.~~gif~~|left|200px]]

[[Image:1pwo.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka~~'''~~

===Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka===

~~==Overview==~~

<!--

~~Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2~~, ~~MiPLA3 and MiPLA4~~, ~~which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is~~ the ~~most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds~~ to the ~~rabbit M-type receptor with high affinity~~. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. ~~The C-termini of the medium (MiPLA4~~) ~~and highly potent (MiPLA2) isoenzyme molecules cluster together~~, ~~forming a highly exposed area. A BLAST search using~~ the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.

The line below this paragraph, {{ABSTRACT_PUBMED_15720395}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15720395 is the PubMed ID number.

-->

==About this Structure==

Line 25:

Line 29:

[[Category: Pancreatic loop]]

[[Category: Phospholipase a2]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 05~~:34:22 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:57:52 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1pwo.gif|left|200px]]
+{{Seed}}
+[[Image:1pwo.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1pwo|  PDB=1pwo  |  SCENE=  }}
-'''Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka'''
+===Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka===
-==Overview==
+<!--
-Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. The C-termini of the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster together, forming a highly exposed area. A BLAST search using the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
+The line below this paragraph, {{ABSTRACT_PUBMED_15720395}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15720395 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15720395}}
 ==About this Structure==
@@ Line 25: / Line 29: @@
 [[Category: Pancreatic loop]]
 [[Category: Phospholipase a2]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:34:22 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:57:52 2008''