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| '''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
| | ===Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe=== |
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| ==Overview==
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| A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15224334}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15224334 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15224334}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Cupredoxin]] | | [[Category: Cupredoxin]] |
| [[Category: Nmr probe]] | | [[Category: Nmr probe]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:37:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:52:57 2008'' |
