1q4k: Difference between revisions

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[[Image:1q4k.jpg|left|200px]]
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[[Image:1q4k.png|left|200px]]


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{{STRUCTURE_1q4k|  PDB=1q4k  |  SCENE=  }}  
{{STRUCTURE_1q4k|  PDB=1q4k  |  SCENE=  }}  


'''The polo-box domain of Plk1 in complex with a phospho-peptide'''
===The polo-box domain of Plk1 in complex with a phospho-peptide===




==Overview==
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Human polo-like kinase Plk1 localizes to the centrosomes, kinetochores and central spindle structures during mitosis. It plays an essential role in promoting mitosis and cytokinesis through phosphorylation of a number of different substrates. Kinase activity is regulated by a conserved C-terminal domain, termed the polo box domain (PBD), which acts both as an autoinhibitory domain and as a subcellular localization domain. We have determined the crystal structure of Plk1 PBD (residues 367-603) to 2.2 A resolution and the structure of a phospho-peptide-PBD (residues 345-603) complex to 2.3 A resolution. The two polo boxes of the PBD exhibit identical folds based on a six-stranded beta-sheet and an alpha-helix, despite only 12% sequence identity. The phospho-peptide binds at a site between the two polo boxes. It makes a short antiparallel beta-sheet connection and critical contacts to residues Trp414, Leu490, His538 and Lys540. Most of these residues had been shown to be important for biological activity through mutational studies. The results provide an explanation for phospho-peptide recognition and create the basis for new functional studies.
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{{ABSTRACT_PUBMED_14592974}}


==About this Structure==
==About this Structure==
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[[Category: Sinclair, J.]]
[[Category: Sinclair, J.]]
[[Category: Six-stranded anti-parallel beta sheet with one alpha helix]]
[[Category: Six-stranded anti-parallel beta sheet with one alpha helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:51:34 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:12:39 2008''

Revision as of 15:12, 27 July 2008

File:1q4k.png

Template:STRUCTURE 1q4k

The polo-box domain of Plk1 in complex with a phospho-peptide

Template:ABSTRACT PUBMED 14592974

About this Structure

1Q4K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex., Cheng KY, Lowe ED, Sinclair J, Nigg EA, Johnson LN, EMBO J. 2003 Nov 3;22(21):5757-68. PMID:14592974

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