8y1l: Difference between revisions
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==Cryo-EM structure of human N-terminally bound ATG9A-ATG2A-WIPI4 complex== | |||
<StructureSection load='8y1l' size='340' side='right'caption='[[8y1l]], [[Resolution|resolution]] 7.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8y1l]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y1L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.05Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y1l OCA], [https://pdbe.org/8y1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y1l RCSB], [https://www.ebi.ac.uk/pdbsum/8y1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y1l ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ATG9A_HUMAN ATG9A_HUMAN] Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.<ref>PMID:16940348</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Autophagy is characterized by the formation of double-membrane vesicles called autophagosomes. Autophagy-related proteins (ATGs) 2A and 9A have an essential role in autophagy by mediating lipid transfer and re-equilibration between membranes for autophagosome formation. Here we report the cryo-electron microscopy structures of human ATG2A in complex with WD-repeat protein interacting with phosphoinositides 4 (WIPI4) at 3.2 A and the ATG2A-WIPI4-ATG9A complex at 7 A global resolution. On the basis of molecular dynamics simulations, we propose a mechanism of lipid extraction from the donor membranes. Our analysis revealed 3:1 stoichiometry of the ATG9A-ATG2A complex, directly aligning the ATG9A lateral pore with ATG2A lipid transfer cavity, and an interaction of the ATG9A trimer with both the N-terminal and the C-terminal tip of rod-shaped ATG2A. Cryo-electron tomography of ATG2A liposome-binding states showed that ATG2A tethers lipid vesicles at different orientations. In summary, this study provides a molecular basis for the growth of the phagophore membrane and lends structural insights into spatially coupled lipid transport and re-equilibration during autophagosome formation. | |||
Structural basis for lipid transfer by the ATG2A-ATG9A complex.,Wang Y, Dahmane S, Ti R, Mai X, Zhu L, Carlson LA, Stjepanovic G Nat Struct Mol Biol. 2024 Aug 22. doi: 10.1038/s41594-024-01376-6. PMID:39174844<ref>PMID:39174844</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8y1l" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Stjepanovic G]] | |||
[[Category: Wang Y]] | |||
