|
|
| Line 1: |
Line 1: |
| [[Image:1q8f.gif|left|200px]] | | {{Seed}} |
| | [[Image:1q8f.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1q8f| PDB=1q8f | SCENE= }} | | {{STRUCTURE_1q8f| PDB=1q8f | SCENE= }} |
|
| |
|
| '''Crystal Structure of the E.coli pyrimidine nucleoside hydrolase yeiK'''
| | ===Crystal Structure of the E.coli pyrimidine nucleoside hydrolase yeiK=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15130467}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15130467 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15130467}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 27: |
Line 31: |
| [[Category: Nh-fold]] | | [[Category: Nh-fold]] |
| [[Category: Open alpha-beta structure]] | | [[Category: Open alpha-beta structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:59:30 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:43:54 2008'' |
