1qa5: Difference between revisions

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[[Image:1qa5.jpg|left|200px]]
{{Seed}}
[[Image:1qa5.png|left|200px]]


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{{STRUCTURE_1qa5|  PDB=1qa5  |  SCENE=  }}  
{{STRUCTURE_1qa5|  PDB=1qa5  |  SCENE=  }}  


'''MYRISTOYLATED HIV-1 NEF ANCHOR DOMAIN, NMR, 2 STRUCTURES'''
===MYRISTOYLATED HIV-1 NEF ANCHOR DOMAIN, NMR, 2 STRUCTURES===




==Overview==
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Negative factor (Nef) is a regulatory myristoylated protein of human immunodeficiency virus (HIV) that has a two-domain structure consisting of an anchor domain and a core domain separated by a specific cleavage site of the HIV proteases. For structural analysis, the HIV-1 Nef anchor domain (residues 2-57) was synthesized with a myristoylated and non-myristoylated N terminus. The structures of the two peptides were studied by1H NMR spectroscopy and a structural model was obtained by restrained molecular dynamic simulations. The non-myristoylated peptide does not have a unique, compactly folded structure but occurs in a relatively extended conformation. The only rather well-defined canonical secondary structure element is a short two-turn alpha-helix (H2) between Arg35 and Gly41. A tendency for another helical secondary structure element (H1) can be observed for the arginine-rich region (Arg17 to Arg22). Myristoylation of the N-terminal glycine residue leads to stabilization of both helices, H1 and H2. The first helix in the arginine-rich region is stabilized by the myristoylation and now contains residues Pro14 to Arg22. The second helix appears to be better defined and to contain more residues (Ala33 to Gly41) than in the absence of myristoylation. In addition, the hydrophobic N-terminal myristic acid residue interacts closely with the side-chain of Trp5 and thereby forms a loop with Gly2, Gly3 and Lys4 in the kink region. This interaction could possibly be disturbed by phosphorylation of a nearby serine residue, and modifiy the characteristic membrane interactions of the HIV-1 Nef anchor domain.
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{{ABSTRACT_PUBMED_10339411}}


==About this Structure==
==About this Structure==
1QA5 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QA5 OCA].  
1QA5 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QA5 OCA].  


==Reference==
==Reference==
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[[Category: Negative factor]]
[[Category: Negative factor]]
[[Category: Regulatory factor]]
[[Category: Regulatory factor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:03:28 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:58:55 2008''

Revision as of 04:59, 28 July 2008

File:1qa5.png

Template:STRUCTURE 1qa5

MYRISTOYLATED HIV-1 NEF ANCHOR DOMAIN, NMR, 2 STRUCTURES

Template:ABSTRACT PUBMED 10339411

About this Structure

1QA5 is a Single protein structure. Full experimental information is available from OCA.

Reference

Structure of the anchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein., Geyer M, Munte CE, Schorr J, Kellner R, Kalbitzer HR, J Mol Biol. 1999 May 28;289(1):123-38. PMID:10339411

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