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| [[Image:1qjm.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1qjm| PDB=1qjm | SCENE= }} | | {{STRUCTURE_1qjm| PDB=1qjm | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF A COMPLEX OF LACTOFERRIN WITH A LANTHANIDE ION (SM3+) AT 3.4 ANSTROM RESOLUTION'''
| | ===CRYSTAL STRUCTURE OF A COMPLEX OF LACTOFERRIN WITH A LANTHANIDE ION (SM3+) AT 3.4 ANSTROM RESOLUTION=== |
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| ==Overview==
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| Lactoferrin is an important member of the transferrin family. A characteristic property of transferrins is their ability to bind very tightly (K(app) approximately/= 10(20)) but reversibly two Fe(3+) ions. The structural consequences of binding a metal other than Fe(3+) have been examined by crystallographic analysis at 3.4 A resolution of mare samarium-lactoferrin (Sm(2)Lf). The structure was refined to an R factor of 0.219 for 8776 reflections in the resolution range 17.0-3.4 A. The samarium geometry (distorted octahedral coordination) is similar in both lobes. However, the anion interactions are quite different in the two lobes. In the N lobe, the anion is able to form only two hydrogen bonds instead of the four observed in the C lobe of Sm(2)Lf and the six observed in Fe(2)Lf. This is because Arg121, Thr117 and Gly124 have moved away from the anion as a consequence of the binding of the Sm(3+) ion. The protein ligands in the binding cleft of Sm(2)Lf show large displacements, but the overall protein structure remains the same. The binding of Sm(3+) by lactoferrin shows that the protein is capable of sequestering ions of different sizes and charges, though with reduced affinity. This conclusion should be true of other transferrins also.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10531475}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10531475 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10531475}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lactoferrin]] | | [[Category: Lactoferrin]] |
| [[Category: Transferrin]] | | [[Category: Transferrin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:21:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:08:54 2008'' |
