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[[Image:1ql5.gif|left|200px]]
{{Seed}}
[[Image:1ql5.png|left|200px]]


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{{STRUCTURE_1ql5|  PDB=1ql5  |  SCENE=  }}  
{{STRUCTURE_1ql5|  PDB=1ql5  |  SCENE=  }}  


'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''
===DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT===




==Overview==
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The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T--&gt;A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%.
The line below this paragraph, {{ABSTRACT_PUBMED_10734199}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10734199 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10734199}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL5 OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL5 OCA].  


==Reference==
==Reference==
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[[Category: Mutagenesis]]
[[Category: Mutagenesis]]
[[Category: Nmr]]
[[Category: Nmr]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:24:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:52:52 2008''

Revision as of 07:52, 29 July 2008

File:1ql5.png

Template:STRUCTURE 1ql5

DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT

Template:ABSTRACT PUBMED 10734199

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:10734199

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