|
|
| Line 1: |
Line 1: |
| [[Image:1qml.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1qml.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1qml| PDB=1qml | SCENE= }} | | {{STRUCTURE_1qml| PDB=1qml | SCENE= }} |
|
| |
|
| '''HG COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE'''
| | ===HG COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| MAD experiments attempting to solve the structure of 5--aminolaevulinic acid dehydratase using Zn and Pb edges are described. The data obtained proved insufficient for a complete structure solution but were invaluable in subsequent identification of metal-binding sites using anomalous difference Fourier analyses once the structure of the enzyme had been solved. These sites include the highly inhibitory substitution of an enzymic cofactor Zn(2+) ion by Pb(2+) ions, which represents a major contribution towards understanding the molecular basis of lead poisoning. The MAD data collected at the Pb edge were also used with isomorphous replacement data from the same Pb co-crystal and a Hg co-crystal to provide the first delineation of the enzyme's quaternary structure. In this MADIR analysis, the Hg co-crystal data were treated as native data. Anomalous difference Fouriers were again used, revealing that Hg(2+) had substituted for the same Zn(2+) cofactor ion as had Pb(2+), a finding of fundamental importance for the understanding of mercury poisoning. In addition, Pt(2+) ions were found to bind at the same place in the structure. The refined structures of the Pb- and the Hg-complexed enzymes are presented at 2.5 and 3.0 A resolution, respectively.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10739915}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10739915 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_10739915}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 32: |
Line 36: |
| [[Category: Tetrapyrrole synthesis]] | | [[Category: Tetrapyrrole synthesis]] |
| [[Category: Tim barrel]] | | [[Category: Tim barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:27:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:50:27 2008'' |
