1ss6: Difference between revisions
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New page: left|200px<br /> <applet load="1ss6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ss6" /> '''Solution structure of SEP domain from human... |
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[[Image:1ss6.gif|left|200px]]<br /> | [[Image:1ss6.gif|left|200px]]<br /><applet load="1ss6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''Solution structure of SEP domain from human p47'''<br /> | '''Solution structure of SEP domain from human p47'''<br /> | ||
==Overview== | ==Overview== | ||
The solution structure of the human p47 SEP domain in a construct | The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171-270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains' function was formulated and pursued in binding experiments with cysteine proteases. The SEP domain was found to be a reversible competitive inhibitor of cathepsin L with a Ki of 1.5 microM. The binding of G1-S2-p47(171-270) to cathepsin L was mapped by biochemical assays and the binding interface was investigated by NMR chemical shift perturbation experiments. | ||
==About this Structure== | ==About this Structure== | ||
1SS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1SS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SS6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ball, L | [[Category: Ball, L J.]] | ||
[[Category: Buessow, K.]] | [[Category: Buessow, K.]] | ||
[[Category: Labudde, D.]] | [[Category: Labudde, D.]] | ||
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[[Category: nmr; p47; sep]] | [[Category: nmr; p47; sep]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:04:38 2008'' | ||
