8s6h: Difference between revisions
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==Cryo-EM Structure of the R388 plasmid conjugative pilus reveals a helical polymer characterised by an unusual pilin/phospholipid binary complex== | |||
<StructureSection load='8s6h' size='340' side='right'caption='[[8s6h]], [[Resolution|resolution]] 3.39Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8s6h]] is a 69 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_HB101 Escherichia coli HB101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8S6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8S6H FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.39Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8s6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8s6h OCA], [https://pdbe.org/8s6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8s6h RCSB], [https://www.ebi.ac.uk/pdbsum/8s6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8s6h ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O50328_ECOLX O50328_ECOLX] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial conjugation is a process by which DNA is transferred unidirectionally from a donor cell to a recipient cell. It is the main means by which antibiotic resistance genes spread among bacterial populations. It is crucially dependent upon the elaboration of an extracellular appendage, termed "pilus," by a large double-membrane-spanning secretion system termed conjugative "type IV secretion system." Here we present the structure of the conjugative pilus encoded by the R388 plasmid. We demonstrate that, as opposed to all conjugative pili produced so far for cryoelectron microscopy (cryo-EM) structure determination, the conjugative pilus encoded by the R388 plasmid is greatly stimulated by the presence of recipient cells. Comparison of its cryo-EM structure with existing conjugative pilus structures highlights a number of important differences between the R388 pilus structure and that of its homologs, the most prominent being the highly distinctive conformation of its bound lipid. | |||
Cryo-EM structure of the R388 plasmid conjugative pilus reveals a helical polymer characterized by an unusual pilin/phospholipid binary complex.,Vadakkepat AK, Xue S, Redzej A, Smith TK, Ho BT, Waksman G Structure. 2024 Jul 8:S0969-2126(24)00227-2. doi: 10.1016/j.str.2024.06.009. PMID:39002540<ref>PMID:39002540</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8s6h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli HB101]] | |||
[[Category: Large Structures]] | |||
[[Category: Redzej A]] | |||
[[Category: Vadakkepat AK]] | |||
[[Category: Waksman G]] | |||
Latest revision as of 06:34, 24 July 2024
Cryo-EM Structure of the R388 plasmid conjugative pilus reveals a helical polymer characterised by an unusual pilin/phospholipid binary complex
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