1r37: Difference between revisions

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[[Image:1r37.jpg|left|200px]]
{{Seed}}
[[Image:1r37.png|left|200px]]


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{{STRUCTURE_1r37|  PDB=1r37  |  SCENE=  }}  
{{STRUCTURE_1r37|  PDB=1r37  |  SCENE=  }}  


'''Alcohol dehydrogenase from sulfolobus solfataricus complexed with NAD(H) and 2-ethoxyethanol'''
===Alcohol dehydrogenase from sulfolobus solfataricus complexed with NAD(H) and 2-ethoxyethanol===




==Overview==
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The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that this medium chain ADH undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface. The extent of domain closure is similar to that observed for the classical horse liver ADH, although some differences are found which can be related to the different oligomeric states of the enzymes. Compared to its apo form, the SsADH ternary complex shows a change in the ligation state of the active site zinc ion which is no longer bound to Glu69, providing additional evidence of the dynamic role played by the conserved glutamate residue in ADHs. In addition, the structure presented here allows the identification of the substrate site and hence of the residues that are important in the binding of both the substrate and the coenzyme.
The line below this paragraph, {{ABSTRACT_PUBMED_14661950}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_14661950}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus., Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, Biochemistry. 2003 Dec 16;42(49):14397-407. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14661950 14661950]
Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus., Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, Biochemistry. 2003 Dec 16;42(49):14397-407. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14661950 14661950]
Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution., Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, J Mol Biol. 2002 Apr 26;318(2):463-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12051852 12051852]
Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity., Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, FEBS Lett. 2003 Mar 27;539(1-3):14-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12650918 12650918]
[[Category: Alcohol dehydrogenase]]
[[Category: Alcohol dehydrogenase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Holoform]]
[[Category: Holoform]]
[[Category: Zinc]]
[[Category: Zinc]]
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