1tgh: Difference between revisions

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[[Image:1tgh.gif|left|200px]]<br />

[[Image:1tgh.gif|left|200px]]<br /><applet load="1tgh" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1tgh" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1tgh, resolution 2.900Å" />

'''TATA BINDING PROTEIN (TBP)/DNA COMPLEX'''<br />

==Overview==

The crystal structure of a complex of human TATA-binding protein with, TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses, from Saccharomyces cerevisiae and Arabidopsis thaliana. Special insight, into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a, particularly large minor groove ligand. This point of view provides, explanations for: (1) why T.A base-pairs are required rather than C.G; (2), why an alternation of T and A bases is needed; (3) how TBP recognizes the, upstream and downstream ends of the TATA box in order to bind properly;, and (4) why the second half of the TATA box can be more variable than the, first.

The crystal structure of a complex of human TATA-binding protein with TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses from Saccharomyces cerevisiae and Arabidopsis thaliana. Special insight into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a particularly large minor groove ligand. This point of view provides explanations for: (1) why T.A base-pairs are required rather than C.G; (2) why an alternation of T and A bases is needed; (3) how TBP recognizes the upstream and downstream ends of the TATA box in order to bind properly; and (4) why the second half of the TATA box can be more variable than the first.

==Disease==

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==About this Structure==

1TGH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1TGH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1TGH OCA].

1TGH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1TGH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGH OCA].

==Reference==

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[[Category: Single protein]]

[[Category: TATA-Binding Protein]]

[[Category: Dickerson, R.E.]]

[[Category: Dickerson, R E.]]

[[Category: Juo, Z.S.]]

[[Category: Juo, Z S.]]

[[Category: dna]]

[[Category: dna binding protein]]

[[Category: transcription factor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 19~~:24:~~37 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1tgh.gif|left|200px]]<br />
+[[Image:1tgh.gif|left|200px]]<br /><applet load="1tgh" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1tgh" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1tgh, resolution 2.900&Aring;" />
 '''TATA BINDING PROTEIN (TBP)/DNA COMPLEX'''<br />
 ==Overview==
-The crystal structure of a complex of human TATA-binding protein with, TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses, from Saccharomyces cerevisiae and Arabidopsis thaliana. Special insight, into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a, particularly large minor groove ligand. This point of view provides, explanations for: (1) why T.A base-pairs are required rather than C.G; (2), why an alternation of T and A bases is needed; (3) how TBP recognizes the, upstream and downstream ends of the TATA box in order to bind properly;, and (4) why the second half of the TATA box can be more variable than the, first.
+The crystal structure of a complex of human TATA-binding protein with TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses from Saccharomyces cerevisiae and Arabidopsis thaliana. Special insight into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a particularly large minor groove ligand. This point of view provides explanations for: (1) why T.A base-pairs are required rather than C.G; (2) why an alternation of T and A bases is needed; (3) how TBP recognizes the upstream and downstream ends of the TATA box in order to bind properly; and (4) why the second half of the TATA box can be more variable than the first.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-TGH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1TGH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TGH OCA].
+TGH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1TGH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb67_1.html TATA-Binding Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGH OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Single protein]]
 [[Category: TATA-Binding Protein]]
-[[Category: Dickerson, R.E.]]
+[[Category: Dickerson, R E.]]
-[[Category: Juo, Z.S.]]
+[[Category: Juo, Z S.]]
 [[Category: dna]]
 [[Category: dna binding protein]]
 [[Category: transcription factor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:24:37 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:15 2008''