1tpg: Difference between revisions
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New page: left|200px<br /> <applet load="1tpg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tpg" /> '''F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TI... |
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[[Image:1tpg.gif|left|200px]]<br /> | [[Image:1tpg.gif|left|200px]]<br /><applet load="1tpg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1tpg" /> | caption="1tpg" /> | ||
'''F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95, REPRESENTATIVE STRUCTURE)'''<br /> | '''F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95, REPRESENTATIVE STRUCTURE)'''<br /> | ||
==Overview== | ==Overview== | ||
BACKGROUND: The thrombolytic serine protease tissue-type plasminogen | BACKGROUND: The thrombolytic serine protease tissue-type plasminogen activator (t-PA) is a classical modular protein consisting of three types of domain in addition to the serine protease domain: F1 (homologous to fibronectin type I); G (epidermal growth factor-like) and kringle. Biochemical data suggest that the F1 and G modules play a major role in the binding of t-PA to fibrin and to receptors on hepatocytes. RESULTS: We have derived the solution structure of the F1 and G pair of modules from t-PA by two- and three-dimensional NMR techniques, in combination with dynamical simulated annealing calculations. We have also obtained information about the molecule's backbone dynamics through measurement of amide 15N relaxation parameters. CONCLUSIONS: Although the F1 and G modules each adopt their expected tertiary structure, the modules interact intimately to bury a hydrophobic core, and the inter-module linker makes up the third strand of the G module's major beta-sheet. The new structural results allow the interpretation of earlier mutational data relevant to fibrin-binding and hepatocyte-receptor binding. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1TPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/T-plasminogen_activator T-plasminogen activator], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.68 3.4.21.68] Full crystallographic information is available from [http:// | 1TPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/T-plasminogen_activator T-plasminogen activator], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.68 3.4.21.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: T-plasminogen activator]] | [[Category: T-plasminogen activator]] | ||
[[Category: Campbell, I | [[Category: Campbell, I D.]] | ||
[[Category: Downing, A | [[Category: Downing, A K.]] | ||
[[Category: Driscoll, P | [[Category: Driscoll, P C.]] | ||
[[Category: Dudgeon, T | [[Category: Dudgeon, T J.]] | ||
[[Category: Smith, B | [[Category: Smith, B O.]] | ||
[[Category: plasminogen activation]] | [[Category: plasminogen activation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:15:59 2008'' | ||
