1rdy: Difference between revisions

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[[Image:1rdy.jpg|left|200px]]
{{Seed}}
[[Image:1rdy.png|left|200px]]


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{{STRUCTURE_1rdy|  PDB=1rdy  |  SCENE=  }}  
{{STRUCTURE_1rdy|  PDB=1rdy  |  SCENE=  }}  


'''T-STATE STRUCTURE OF THE ARG 243 TO ALA MUTANT OF PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI'''
===T-STATE STRUCTURE OF THE ARG 243 TO ALA MUTANT OF PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI===




==Overview==
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The active site of pig kidney fructose-1,6-bisphosphatase (EC 3.1.3.11) is shared between subunits, Arg-243 of one chain interacting with fructose-1,6-bisphosphate or fructose-2,6-bisphosphate in the active site of an adjacent chain. In this study, we present the X-ray structures of the mutant version of the enzyme with Arg-243 replaced by alanine, crystallized in both T and R allosteric states. Kinetic characteristics of the altered enzyme showed the magnesium binding and inhibition by AMP differed slightly; affinity for the substrate fructose-1,6-bisphosphate was reduced 10-fold and affinity for the inhibitor fructose-2,6-bisphosphate was reduced 1,000-fold (Giroux E, Williams MK, Kantrowitz ER, 1994, J Biol Chem 269:31404-31409). The X-ray structures show no major changes in the organization of the active site compared with wild-type enzyme, and the structures confirm predictions of molecular dynamics simulations involving Lys-269 and Lys-274. Comparison of two independent models of the T form structures have revealed small but significant changes in the conformation of the bound AMP molecules and small reorganization of the active site correlated with the presence of the inhibitor. The differences in kinetic properties of the mutant enzyme indicate the key importance of Arg-243 in the function of fructose-1,6-bisphosphatase. Calculations using the X-ray structures of the Arg-243--&gt;Ala enzyme suggest that the role of Arg-243 in the wild-type enzyme is predominantly electrostatic in nature.
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{{ABSTRACT_PUBMED_8844845}}


==About this Structure==
==About this Structure==
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[[Category: Stec, B.]]
[[Category: Stec, B.]]
[[Category: Hydrolase r243a mutant in the t-state]]
[[Category: Hydrolase r243a mutant in the t-state]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:22:47 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:30:08 2008''

Revision as of 14:30, 27 July 2008

File:1rdy.png

Template:STRUCTURE 1rdy

T-STATE STRUCTURE OF THE ARG 243 TO ALA MUTANT OF PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI

Template:ABSTRACT PUBMED 8844845

About this Structure

1RDY is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structures of the active site mutant (Arg-243-->Ala) in the T and R allosteric states of pig kidney fructose-1,6-bisphosphatase expressed in Escherichia coli., Stec B, Abraham R, Giroux E, Kantrowitz ER, Protein Sci. 1996 Aug;5(8):1541-53. PMID:8844845

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