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| {{STRUCTURE_1ri8| PDB=1ri8 | SCENE= }} | | {{STRUCTURE_1ri8| PDB=1ri8 | SCENE= }} |
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| '''Crystal Structure of the Camelid Single Domain Antibody 1D2L19 in complex with Hen Egg White Lysozyme'''
| | ===Crystal Structure of the Camelid Single Domain Antibody 1D2L19 in complex with Hen Egg White Lysozyme=== |
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| ==Overview==
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| A central paradigm in immunology states that successful generation of high affinity antibodies necessitates an immense primary repertoire of antigen-combining sites. Much of the diversity of this repertoire is provided by varying one antigen binding loop, created by inserting randomly a D (diversity) gene out of a small pool between the V and J genes. It is therefore assumed that any particular D-encoded region surrounded by different V and J regions adopts a different conformation. We have solved the structure of two lysozyme-specific variable domains of heavy-chain antibodies isolated from two strictly unrelated dromedaries. These antibodies recombined identical D gene sequences to different V and J precursors with significant variance in their V(D)J junctions. Despite these large differences, the D-encoded loop segments adopt remarkably identical architectures, thus directing the antibodies toward identical epitopes. Furthermore, a striking convergent maturation process occurred in the V region, adapting both binders for their sub-nanomolar affinity association with lysozyme. Hence, on a structural level, humoral immunity may rely more on well developed maturation and selection systems than on the acquisition of large primary repertoires.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15659390}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15659390 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15659390}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Immunoglobulin fold]] | | [[Category: Immunoglobulin fold]] |
| [[Category: Vhh-lysozyme complex]] | | [[Category: Vhh-lysozyme complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:31:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:00:37 2008'' |
