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| {{STRUCTURE_1rk4| PDB=1rk4 | SCENE= }} | | {{STRUCTURE_1rk4| PDB=1rk4 | SCENE= }} |
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| '''Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1'''
| | ===Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1=== |
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| ==Overview==
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| Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_14613939}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14613939 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14613939}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Glutathione-s-tranferase superfamily]] | | [[Category: Glutathione-s-tranferase superfamily]] |
| [[Category: Redox-controlled structural transition]] | | [[Category: Redox-controlled structural transition]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:35:45 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:53:55 2008'' |
