1rkl: Difference between revisions

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[[Image:1rkl.gif|left|200px]]
{{Seed}}
[[Image:1rkl.png|left|200px]]


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{{STRUCTURE_1rkl|  PDB=1rkl  |  SCENE=  }}  
{{STRUCTURE_1rkl|  PDB=1rkl  |  SCENE=  }}  


'''NMR structure of yeast oligosaccharyltransferase subunit Ost4p'''
===NMR structure of yeast oligosaccharyltransferase subunit Ost4p===




==Overview==
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N-glycosylation of proteins is an essential, highly conserved modification reaction that occurs in all eukaryotes and some prokaryotes. This process is catalyzed by oligosaccharyltransferase (OT), a multisubunit enzyme localized in the endoplasmic reticulum. Complete loss of N-glycosylation is lethal in all organisms. In Saccharomyces cerevisiae, OT is composed of nine nonidentical membrane proteins. Here, we report the atomic structure of an OT subunit from S. cerevisiae, Ost4p. This unusually small membrane protein containing only 36 residues folds into a well formed, kinked helix in the model-membrane solvent system used in this study. The residues critical for the OT activity and the stability of Stt3p-Ost4p-Ost3p subcomplex are located in helix alpha2, the larger cytosolic half of this kinked helix. The residues known to disrupt Ost4p-Stt3p complex form a well defined ridge in the 3D structure. Taking together prior mutational studies and the NMR structure of Ost4p, we propose that in the OT complex Stt3p is packed against the alpha 2-helix of Ost4p by using a "ridges-into-grooves" model, with Met-18, Leu-21, and Ile-24 as the packing interface on one face, whereas Ost3p is involved in interactions with Met-19, Thr-20, Ile-22, and Val-23 on the other face.
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{{ABSTRACT_PUBMED_15001703}}


==About this Structure==
==About this Structure==
1RKL is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKL OCA].  
1RKL is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKL OCA].  


==Reference==
==Reference==
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[[Category: Zubkov, S.]]
[[Category: Zubkov, S.]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:36:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:43:34 2008''

Revision as of 21:43, 27 July 2008

File:1rkl.png

Template:STRUCTURE 1rkl

NMR structure of yeast oligosaccharyltransferase subunit Ost4p

Template:ABSTRACT PUBMED 15001703

About this Structure

1RKL is a Single protein structure. Full experimental information is available from OCA.

Reference

Structural basis for the function of a minimembrane protein subunit of yeast oligosaccharyltransferase., Zubkov S, Lennarz WJ, Mohanty S, Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3821-6. Epub 2004 Mar 4. PMID:15001703

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