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| [[Image:1rl0.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1rl0| PDB=1rl0 | SCENE= }} | | {{STRUCTURE_1rl0| PDB=1rl0 | SCENE= }} |
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| '''Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30'''
| | ===Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30=== |
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| ==Overview==
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| Ribosome inactivating proteins (RIPs) are plant proteins with enzymatic activity identified as rRNA N-glycosidase (EC 3.2.2.22), which cleaves the N-glycosidic bond of a specific adenine on the ricin/sarcin region of rRNA, thus causing inhibition of protein synthesis. They also depurinate extensively DNA and other polynucleotides. The three-dimensional structure of dianthin 30, a type 1 (single-chain) RIP of Dianthus caryophyllus (leaves), is now described at 1.4 angstroms, a resolution never achieved before for any RIP. The fold typical of RIPs is conserved, despite some differences in the loop regions. The general structure comparison by superimposed alpha-carbon (249 atoms) and the sequence alignment by structure for dianthin 30 and saporin-S6 give a root mean square deviation of 0.625 angstroms. Despite the differences reported for the biological activities of the two RIPs, their structures fit quite well and both show a protein segment containing strands beta7, beta8, and beta9 shorter than other RIPs. However, the surface electrostatic potential in the active site region neatly distinguishes dianthin 30 from saporin-S6. The possible relationship between the charge distribution and the behavior of the proteins toward different substrates is discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15681236}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15681236 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15681236}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Alpha and beta region]] | | [[Category: Alpha and beta region]] |
| [[Category: Mixed beta-sheet]] | | [[Category: Mixed beta-sheet]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:37:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:14:10 2008'' |
