1rlt: Difference between revisions

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[[Image:1rlt.jpg|left|200px]]
{{Seed}}
[[Image:1rlt.png|left|200px]]


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{{STRUCTURE_1rlt|  PDB=1rlt  |  SCENE=  }}  
{{STRUCTURE_1rlt|  PDB=1rlt  |  SCENE=  }}  


'''Transition State Analogue of ybiV from E. coli K12'''
===Transition State Analogue of ybiV from E. coli K12===




==Overview==
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The protein YbiV from Escherichia coli K12 MG1655 is a hypothetical protein with sequence homology to the haloacid dehalogenase (HAD) superfamily of proteins. Although numerous members of this family have been identified, the functions of few are known. Using the crystal structure, sequence analysis, and biochemical assays, we have characterized YbiV as a HAD phosphatase. The crystal structure of YbiV reveals a two-domain protein, one with the characteristic HAD hydrolase fold, the other an inserted alpha/beta fold. In an effort to understand the mechanism, we also solved and report the structures of YbiV in complex with beryllofluoride (BeF3-) and aluminum trifluoride (AlF3), which have been shown to mimic the phosphorylated intermediate and transition state for hydrolysis, respectively, in analogy to other HAD phosphatases. Analysis of the structures reveals the substrate-binding cavity, which is hydrophilic in nature. Both structure and sequence homology indicate YbiV may be a sugar phosphatase, which is supported by biochemical assays that measured the release of free phosphate on a number of sugar-like substrates. We also investigated available genomic and functional data in an effort to determine the physiological substrate.
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{{ABSTRACT_PUBMED_15657928}}


==About this Structure==
==About this Structure==
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[[Category: Phosphatase]]
[[Category: Phosphatase]]
[[Category: Transition-state analogue]]
[[Category: Transition-state analogue]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:39:17 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:12:02 2008''

Revision as of 13:12, 29 July 2008

File:1rlt.png

Template:STRUCTURE 1rlt

Transition State Analogue of ybiV from E. coli K12

Template:ABSTRACT PUBMED 15657928

About this Structure

1RLT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

YbiV from Escherichia coli K12 is a HAD phosphatase., Roberts A, Lee SY, McCullagh E, Silversmith RE, Wemmer DE, Proteins. 2005 Mar 1;58(4):790-801. PMID:15657928

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