Sandbox 323: Difference between revisions
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==Proposed Structure of 3DS8 Protein== | ==Proposed Structure of 3DS8 Protein== | ||
<scene name='10/1043632/3ds8_secondary_structure/1'>3DS8 Secondary Structure</scene> | <scene name='10/1043632/3ds8_secondary_structure/1'>3DS8 Secondary Structure</scene> | ||
The discovery and characterization of the structure and function of the protein 3DS8. | The discovery and characterization of the structure and function of the protein 3DS8. | ||
This project aimed to characterize the function of an unknown protein 3DS8 with a known structure by comparing it to known proteins and subjecting it to different tests in the laboratory. | This project aimed to characterize the function of an unknown protein 3DS8 with a known structure by comparing it to known proteins and subjecting it to different tests in the laboratory. | ||
Different techniques to achieve this goal include protein expression, purification and analysis, kinetics, and computational methods. | Different techniques to achieve this goal include protein expression, purification and analysis, kinetics, and computational methods. | ||
<StructureSection load='3DS8' size='300' side='right' caption='3DS8 Structure' scene=''> | |||
== Function == | == Function == | ||
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The function was not confirmed during wet lab experiments due to protein and reagent complications. | The function was not confirmed during wet lab experiments due to protein and reagent complications. | ||
</StructureSection> | |||
== Experimental == | == Experimental == | ||
The beginning experiments were conducted on molecular docking sites to compare the structure of the 3DS8 active site with known proteins. Using the SPRITE database [1], | The beginning experiments were conducted on molecular docking sites to compare the structure of the 3DS8 active site with known proteins. Using the SPRITE database [1], | ||
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The molecular weight of 3DS8 is proposed to be about 28 kDa. | The molecular weight of 3DS8 is proposed to be about 28 kDa. | ||
==Discussion== | ==Discussion== | ||
