1ucb: Difference between revisions

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[[Image:1ucb.gif|left|200px]]<br />

[[Image:1ucb.gif|left|200px]]<br /><applet load="1ucb" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1ucb" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1ucb, resolution 2.5Å" />

'''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br />

==Overview==

The X-ray structure of the uncomplexed human chimeric Fab' of the, anti-tumor antibody BR96 has been determined at 2.6 A resolution. The, structure has been compared with Lewis Y antigen-complexed structures of, BR96 which were determined previously. The comparison reveals segmental, motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite, its significant main-chain contacts to the carbohydrate antigen. In light, of the uncomplexed chimeric Fab' structure reported here, the previously, observed high mobility of the CL:CH1 domains of the complexed chimeric, BR96 Fab is rationalized as a "swinging" motion approximately about the, axis of the elbow bend.

The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.

==Disease==

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==About this Structure==

1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].

1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].

==Reference==

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[[Category: immunoglobulin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 19~~:33:~~14 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:54 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ucb.gif|left|200px]]<br />
+[[Image:1ucb.gif|left|200px]]<br /><applet load="1ucb" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1ucb" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1ucb, resolution 2.5&Aring;" />
 '''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br />
 ==Overview==
-The X-ray structure of the uncomplexed human chimeric Fab' of the, anti-tumor antibody BR96 has been determined at 2.6 A resolution. The, structure has been compared with Lewis Y antigen-complexed structures of, BR96 which were determined previously. The comparison reveals segmental, motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite, its significant main-chain contacts to the carbohydrate antigen. In light, of the uncomplexed chimeric Fab' structure reported here, the previously, observed high mobility of the CL:CH1 domains of the complexed chimeric, BR96 Fab is rationalized as a "swinging" motion approximately about the, axis of the elbow bend.
+The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].
+UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].
 ==Reference==
@@ Line 26: / Line 25: @@
 [[Category: immunoglobulin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:33:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:54 2008''