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| {{STRUCTURE_1rrq| PDB=1rrq | SCENE= }} | | {{STRUCTURE_1rrq| PDB=1rrq | SCENE= }} |
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| '''MutY adenine glycosylase in complex with DNA containing an A:oxoG pair'''
| | ===MutY adenine glycosylase in complex with DNA containing an A:oxoG pair=== |
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| ==Overview==
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| The genomes of aerobic organisms suffer chronic oxidation of guanine to the genotoxic product 8-oxoguanine (oxoG). Replicative DNA polymerases misread oxoG residues and insert adenine instead of cytosine opposite the oxidized base. Both bases in the resulting A*oxoG mispair are mutagenic lesions, and both must undergo base-specific replacement to restore the original C*G pair. Doing so represents a formidable challenge to the DNA repair machinery, because adenine makes up roughly 25% of the bases in most genomes. The evolutionarily conserved enzyme adenine DNA glycosylase (called MutY in bacteria and hMYH in humans) initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. A central issue concerning MutY function is the mechanism by which A*oxoG mispairs are targeted among the vast excess of A*T pairs. Here we report the use of disulphide crosslinking to obtain high-resolution crystal structures of MutY-DNA lesion-recognition complexes. These structures reveal the basis for recognizing both lesions in the A*oxoG pair and for catalysing removal of the adenine base. | | The line below this paragraph, {{ABSTRACT_PUBMED_14961129}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14961129 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14961129}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Dna repair]] | | [[Category: Dna repair]] |
| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:50:01 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:56:23 2008'' |
