1rw4: Difference between revisions

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[[Image:1rw4.jpg|left|200px]]
{{Seed}}
[[Image:1rw4.png|left|200px]]


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{{STRUCTURE_1rw4|  PDB=1rw4  |  SCENE=  }}  
{{STRUCTURE_1rw4|  PDB=1rw4  |  SCENE=  }}  


'''Nitrogenase Fe protein l127 deletion variant'''
===Nitrogenase Fe protein l127 deletion variant===




==Overview==
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The crystal structure of a nitrogenase Fe protein single site deletion variant reveals a distinctly new conformation of the Fe protein and indicates that, upon binding of MgATP, the Fe protein undergoes a dramatic conformational change that is largely manifested in the rigid-body reorientation of the homodimeric Fe protein subunits with respect to one another. The observed conformational state allows the rationalization of a model of structurally and chemically complementary interactions that occur upon initial complex formation with the MoFe protein component that are distinct from the protein-protein interactions that have been characterized previously for stabilized nitrogenase complexes. The crystallographic results, in combination with complementary UV-visible absorption, EPR, and resonance Raman spectroscopic data, indicate that the [4Fe-4S] cluster of both the Fe protein deletion variant and the native Fe protein in the presence of MgATP can reversibly cycle between a regular cubane-type [4Fe-4S] cluster in the reduced state and a cleaved form involving two [2Fe-2S] fragments in the oxidized state. Resonance Raman studies indicate that this novel cluster conversion is induced by glycerol, and the crystallographic data suggest that glycerol is bound as a bridging bidentate ligand to both [2Fe-2S] cluster fragments in the oxidized state.
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{{ABSTRACT_PUBMED_14967020}}


==About this Structure==
==About this Structure==
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[[Category: Smith, A.]]
[[Category: Smith, A.]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:58:31 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:16:09 2008''

Revision as of 17:16, 27 July 2008

File:1rw4.png

Template:STRUCTURE 1rw4

Nitrogenase Fe protein l127 deletion variant

Template:ABSTRACT PUBMED 14967020

About this Structure

1RW4 is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein., Sen S, Igarashi R, Smith A, Johnson MK, Seefeldt LC, Peters JW, Biochemistry. 2004 Feb 24;43(7):1787-97. PMID:14967020

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