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| [[Image:1rxe.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1rxe| PDB=1rxe | SCENE= }} | | {{STRUCTURE_1rxe| PDB=1rxe | SCENE= }} |
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| '''ArsC complexed with MNB'''
| | ===ArsC complexed with MNB=== |
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| ==Overview==
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| Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15159594}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15159594 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15159594}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Mnb]] | | [[Category: Mnb]] |
| [[Category: Tnb]] | | [[Category: Tnb]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:01:15 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:06:02 2008'' |
