9b9p: Difference between revisions
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==Crystal structure of Staphylococcus aureus ketol-acid reductoisomerase in complex with Mg2+, and JK-5-114== | |||
<StructureSection load='9b9p' size='340' side='right'caption='[[9b9p]], [[Resolution|resolution]] 3.18Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9b9p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9B9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9B9P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.18Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1AKJ:6-oxidanyl-2-phenyl-4~{H}-[1,3]thiazolo[4,5-d]pyrimidine-5,7-dione'>A1AKJ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9b9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9b9p OCA], [https://pdbe.org/9b9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9b9p RCSB], [https://www.ebi.ac.uk/pdbsum/9b9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9b9p ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A4T9XKD7_STAAU A0A4T9XKD7_STAAU] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.[HAMAP-Rule:MF_00435] | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Staphylococcus aureus]] | |||
[[Category: Guddat LW]] | |||
[[Category: Kurz JL]] | |||
[[Category: Lin X]] | |||
Revision as of 07:50, 9 April 2025
Crystal structure of Staphylococcus aureus ketol-acid reductoisomerase in complex with Mg2+, and JK-5-114
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