1ry5: Difference between revisions

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[[Image:1ry5.gif|left|200px]]
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[[Image:1ry5.png|left|200px]]


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{{STRUCTURE_1ry5|  PDB=1ry5  |  SCENE=  }}  
{{STRUCTURE_1ry5|  PDB=1ry5  |  SCENE=  }}  


'''PHOTOSYNTHETIC REACTION CENTER MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN'''
===PHOTOSYNTHETIC REACTION CENTER MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN===




==Overview==
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In the photosynthetic reaction center (RC) from Rhodobacter sphaeroides, the reduction of a bound quinone molecule Q(B) is coupled with proton uptake. When Asp-L213 is replaced by Asn, proton transfer is inhibited. Proton transfer was restored by two second-site revertant mutations, Arg-M233--&gt;Cys and Arg-H177--&gt;His. Kinetic effects of Cd(2+) on proton transfer showed that the entry point in revertant RCs to be the same as in the native RC. The structures of the parental and two revertant RCs were determined at resolutions of 2.10, 1.80, and 2.75 A. From the structures, we were able to delineate alternate proton transfer pathways in the revertants. The main changes occur near Glu-H173, which allow it to substitute for the missing Asp-L213. The electrostatic changes near Glu-H173 cause it to be a good proton donor and acceptor, and the structural changes create a cavity which accommodates water molecules that connect Glu-H173 to other proton transfer components.
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{{ABSTRACT_PUBMED_15062092}}


==About this Structure==
==About this Structure==
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[[Category: Rhodobacter sphaeroide]]
[[Category: Rhodobacter sphaeroide]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:28:19 2008''

Revision as of 12:28, 29 July 2008

File:1ry5.png

Template:STRUCTURE 1ry5

PHOTOSYNTHETIC REACTION CENTER MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN

Template:ABSTRACT PUBMED 15062092

About this Structure

1RY5 is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:15062092

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