1s9s: Difference between revisions

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[[Image:1s9s.gif|left|200px]]
{{Seed}}
[[Image:1s9s.png|left|200px]]


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{{STRUCTURE_1s9s|  PDB=1s9s  |  SCENE=  }}  
{{STRUCTURE_1s9s|  PDB=1s9s  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF MLV PSI SITE'''
===SOLUTION STRUCTURE OF MLV PSI SITE===




==Overview==
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The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.
The line below this paragraph, {{ABSTRACT_PUBMED_15003457}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15003457 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15003457}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA].  


==Reference==
==Reference==
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[[Category: Stem loop]]
[[Category: Stem loop]]
[[Category: U-u mismatch]]
[[Category: U-u mismatch]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:27:44 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:13:59 2008''
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