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| [[Image:1scl.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1scl| PDB=1scl | SCENE= }} | | {{STRUCTURE_1scl| PDB=1scl | SCENE= }} |
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| '''THE SARCIN-RICIN LOOP, A MODULAR RNA'''
| | ===THE SARCIN-RICIN LOOP, A MODULAR RNA=== |
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| ==Overview==
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| The sarcin/ricin loop is a highly conserved sequence found in the RNA of all large ribosomal subunits. The cytotoxins alpha-sarcin and ricin both inactivate ribosomes by cleaving a single bond in that loop. Once it has been attacked, ribosomes no longer interact with elongation factors properly, and translation stops. We have determined the conformation of the sarcin/ricin loop by multinuclear NMR spectroscopy using E73, a 29-nucleotide RNA that has the sarcin/ricin loop sequence and that is sensitive to both toxins in vitro. The sarcin/ricin loop has a compact structure that contains several purine.purine base pairs, a GAGA tetraloop, and a bulged guanosine adjacent to a reverse Hoogsteen A.U pair. It is stabilized by an unusual set of cross-strand base-stacking interactions and imino proton to phosphate oxygen hydrogen bonds. In addition to having interesting structural features, this model explains many of the biochemical observations made about the loop's structure and its reactivity with cytotoxins, and it sheds light on the loop's interactions with elongation factors. | | The line below this paragraph, {{ABSTRACT_PUBMED_8415744}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8415744 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8415744}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCL OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCL OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Rna]] | | [[Category: Rna]] |
| [[Category: Sarcin-ricin loop]] | | [[Category: Sarcin-ricin loop]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:32:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:27:05 2008'' |
