8y9d: Difference between revisions
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==Versatile Aromatic Prenyltransferase auraA in complex with DMAPP and cyclo-(L-Val-L-His)== | |||
<StructureSection load='8y9d' size='340' side='right'caption='[[8y9d]], [[Resolution|resolution]] 2.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8y9d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium Penicillium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y9D FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.45Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1LYE:(3~{S},6~{S})-3-(1~{H}-imidazol-4-ylmethyl)-6-propan-2-yl-piperazine-2,5-dione'>A1LYE</scene>, <scene name='pdbligand=DST:DIMETHYLALLYL+S-THIOLODIPHOSPHATE'>DST</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y9d OCA], [https://pdbe.org/8y9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y9d RCSB], [https://www.ebi.ac.uk/pdbsum/8y9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y9d ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prenylation modifications of natural products play essential roles in chemical diversity and bioactivities, but imidazole modification prenyltransferases are not well investigated. Here, we discover a dimethylallyl tryptophan synthase family prenyltransferase, AuraA, that catalyzes the rare dimethylallylation on the imidazole moiety in the biosynthesis of aurantiamine. Biochemical assays validate that AuraA could accept both cyclo-(L-Val-L-His) and cyclo-(L-Val-DH-His) as substrates, while the prenylation modes are completely different, yielding C2-regular and C5-reverse products, respectively. Cryo-electron microscopy analysis of AuraA and its two ternary complex structures reveal two distinct modes for receptor binding, demonstrating a tolerance for altered orientations of highly similar receptors. The mutation experiments further demonstrate the promiscuity of AuraA towards imidazole-C-dimethylallylation. In this work, we also characterize a case of AuraA mutant-catalyzed dimethylallylation of imidazole moiety, offering available structural insights into the utilization and engineering of dimethylallyl tryptophan synthase family prenyltransferases. | |||
Characterization and structural analysis of a versatile aromatic prenyltransferase for imidazole-containing diketopiperazines.,Wang W, Wang P, Ma C, Li K, Wang Z, Liu Y, Wang L, Zhang G, Che Q, Zhu T, Zhang Y, Li D Nat Commun. 2025 Jan 2;16(1):144. doi: 10.1038/s41467-024-55537-8. PMID:39747040<ref>PMID:39747040</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8y9d" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Penicillium]] | |||
[[Category: Li D]] | |||
[[Category: Wang P]] | |||
[[Category: Wang W]] | |||
[[Category: Zhang Y]] | |||
Latest revision as of 06:23, 5 February 2025
Versatile Aromatic Prenyltransferase auraA in complex with DMAPP and cyclo-(L-Val-L-His)
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