1sf4: Difference between revisions

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[[Image:1sf4.jpg|left|200px]]
{{Seed}}
[[Image:1sf4.png|left|200px]]


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{{STRUCTURE_1sf4|  PDB=1sf4  |  SCENE=  }}  
{{STRUCTURE_1sf4|  PDB=1sf4  |  SCENE=  }}  


'''BINDING OF N,N'-DIACETYLCHITOBIOSE TO HEW LYSOZYME: A POWDER DIFFRACTION STUDY'''
===BINDING OF N,N'-DIACETYLCHITOBIOSE TO HEW LYSOZYME: A POWDER DIFFRACTION STUDY===




==Overview==
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The binding of N-acetylglucosamine oligosaccharides (NAGn, n = 2-6) to hen egg-white lysozyme (HEWL; EC 3.2.1.17) was investigated by X-ray powder diffraction at room temperature. Each NAGn examined was found to bind to lysozyme in rapid-precipitation preparations in 1.0 M NaCl pH 6.0 buffer. The location of each NAGn was easily found from difference Fourier maps generated from structure factors extracted during preliminary Rietveld refinements. Full NAGn-protein structures were subjected to combined Rietveld and stereochemical restraint refinements (Rwp = 2.28-2.59%; Rp = 1.81-2.04%; RF2 = 3.91-5.80%) and revealed binding modes for NAGn that depended on the length of the NAG oligosaccharide. The NAG2 ligand was found in the BC sites in the cleft of HEWL, NAG3 was found to bind in both the ABC and BCD sites in the ratio 35:65 and NAG4 and NAG5 bound to the ABCD and ABCDE sites, respectively, while NAG6 only bound to sites ABCDE, leaving the F site empty with the remaining saccharide ring located in a solvent region adjacent to the A site. All protein powder diffraction patterns in this study consisted of extremely sharp Bragg peaks consistent with approximately 1 microm crystallites that were devoid of line-broadening defects. Details of the stereochemical restraints used in these refinements and their impact on structural validation are also discussed.
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{{ABSTRACT_PUBMED_15608372}}


==About this Structure==
==About this Structure==
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[[Category: Powder diffraction]]
[[Category: Powder diffraction]]
[[Category: Rietveld refinement]]
[[Category: Rietveld refinement]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:37:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:52:25 2008''

Revision as of 19:52, 27 July 2008

File:1sf4.png

Template:STRUCTURE 1sf4

BINDING OF N,N'-DIACETYLCHITOBIOSE TO HEW LYSOZYME: A POWDER DIFFRACTION STUDY

Template:ABSTRACT PUBMED 15608372

About this Structure

1SF4 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Binding of N-acetylglucosamine oligosaccharides to hen egg-white lysozyme: a powder diffraction study., Von Dreele RB, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):22-32. Epub 2004, Dec 17. PMID:15608372

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