1smy: Difference between revisions

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[[Image:1smy.~~gif~~|left|200px]]

[[Image:1smy.png|left|200px]]

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~~'''~~Structural basis for transcription regulation by alarmone ppGpp~~'''~~

===Structural basis for transcription regulation by alarmone ppGpp===

~~==Overview==~~

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~~Guanosine-tetraphosphate (ppGpp) is a major regulator of stringent control~~, ~~an adaptive response of bacteria~~ to ~~amino acid starvation~~. ~~The 2~~.~~7 A resolution structure of the Thermus thermophilus RNA polymerase (RNAP~~) ~~holoenzyme in complex with ppGpp reveals that ppGpp binds to the same site near the active center in both independent RNAP molecules in the crystal but in strikingly distinct orientations. Binding~~ is ~~symmetrical with respect to~~ the ~~two diphosphates of ppGpp and is relaxed with respect to the orientation of the nucleotide base~~. Different modes of ppGpp binding are coupled with asymmetry of the active site configurations. The results suggest that base pairing of ppGpp with cytosines in the nontemplate DNA strand might be an essential component of transcription control by ppGpp. We present experimental evidence highlighting the importance of base-~~specific contacts between ppGpp and specific cytosine residues during both transcription initiation and elongation.~~

The line below this paragraph, {{ABSTRACT_PUBMED_15109491}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15109491 is the PubMed ID number.

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==About this Structure==

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[[Category: Structural genomic]]

[[Category: Transcription regulation]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:54:18 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:20:31 2008''

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-[[Image:1smy.gif|left|200px]]
+{{Seed}}
+[[Image:1smy.png|left|200px]]
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 {{STRUCTURE_1smy|  PDB=1smy  |  SCENE=  }}
-'''Structural basis for transcription regulation by alarmone ppGpp'''
+===Structural basis for transcription regulation by alarmone ppGpp===
-==Overview==
+<!--
-Guanosine-tetraphosphate (ppGpp) is a major regulator of stringent control, an adaptive response of bacteria to amino acid starvation. The 2.7 A resolution structure of the Thermus thermophilus RNA polymerase (RNAP) holoenzyme in complex with ppGpp reveals that ppGpp binds to the same site near the active center in both independent RNAP molecules in the crystal but in strikingly distinct orientations. Binding is symmetrical with respect to the two diphosphates of ppGpp and is relaxed with respect to the orientation of the nucleotide base. Different modes of ppGpp binding are coupled with asymmetry of the active site configurations. The results suggest that base pairing of ppGpp with cytosines in the nontemplate DNA strand might be an essential component of transcription control by ppGpp. We present experimental evidence highlighting the importance of base-specific contacts between ppGpp and specific cytosine residues during both transcription initiation and elongation.
+The line below this paragraph, {{ABSTRACT_PUBMED_15109491}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15109491 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15109491}}
 ==About this Structure==
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 [[Category: Structural genomic]]
 [[Category: Transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:54:18 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:20:31 2008''