1sqj: Difference between revisions

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[[Image:1sqj.gif|left|200px]]
{{Seed}}
[[Image:1sqj.png|left|200px]]


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{{STRUCTURE_1sqj|  PDB=1sqj  |  SCENE=  }}  
{{STRUCTURE_1sqj|  PDB=1sqj  |  SCENE=  }}  


'''Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)'''
===Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)===




==Overview==
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Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH; EC 3.2.1.150) is an exoglucanase that recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain. The X-ray crystal structure of OXG-RCBH determined at 2.2 A resolution reveals a unique feature of this enzyme; OXG-RCBH consists of a tandem repeat of two similar domains, which are both folded into seven-bladed beta-propeller structures. The sequence alignment of the propeller blades, based on the structure, indicates that a weak repeat of the amino acid sequence occurred seven times to construct each domain. There is a cleft that can accommodate the substrate oligosaccharide between the two domains, which is a putative substrate binding subsite. Mutation of either Asp35 or Asp465, located in the putative catalytic center, to Asn resulted in a protein with no detectable catalytic activity, indicating the critical role of these amino acids in catalysis.
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{{ABSTRACT_PUBMED_15242597}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242597 15242597]
Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242597 15242597]
Purification, characterization, cloning, and expression of a novel xyloglucan-specific glycosidase, oligoxyloglucan reducing end-specific cellobiohydrolase., Yaoi K, Mitsuishi Y, J Biol Chem. 2002 Dec 13;277(50):48276-81. Epub 2002 Oct 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12374797 12374797]
Crystallization and preliminary X-ray crystallographic study on a xyloglucan-specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase., Yaoi K, Kondo H, Suzuki M, Noro N, Tsuda S, Mitsuishi Y, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1838-9. Epub 2003, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14501131 14501131]
[[Category: Geotrichum sp. m128]]
[[Category: Geotrichum sp. m128]]
[[Category: Oligoxyloglucan reducing-end-specific cellobiohydrolase]]
[[Category: Oligoxyloglucan reducing-end-specific cellobiohydrolase]]
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[[Category: Yaoi, K.]]
[[Category: Yaoi, K.]]
[[Category: Beta-propeller]]
[[Category: Beta-propeller]]
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