9ca0: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 9ca0 is ~~ON HOLD~~

==Human TOP3B-TDRD3 core complex in DNA pre-cleavage state==

~~Authors~~: ~~Yang~~, X., ~~Chen~~, X., ~~Yang~~, W., ~~Pommier, Y~~.

== Structural highlights ==

<table><tr><td colspan='2'>[[9ca0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CA0 OCA]. For a guided tour on the structure components use [https://proteopedia.org/fgij/fg.htm?mol=9CA0 FirstGlance].

~~Description~~: ~~Human TOP3B~~-~~TDRD3 core complex~~ in DNA ~~pre~~-~~cleavage state~~

</td></tr><tr id='method'><td class="sblockLbl">[[Empirical_models|Method:]]</td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.48Å</td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>

[[Category: Chen, X]]

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[https://proteopedia.org/fgij/fg.htm?mol=9ca0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ca0 OCA], [https://pdbe.org/9ca0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ca0 RCSB], [https://www.ebi.ac.uk/pdbsum/9ca0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ca0 ProSAT]</td></tr>

[[Category: ~~Yang, W~~]]

</table>

[[Category: Yang~~, X~~]]

== Function ==

[[Category: ~~Pommier, Y~~]]

[https://www.uniprot.org/uniprot/TOP3B_HUMAN TOP3B_HUMAN] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Large Structures]]

[[Category: Chen X]]

[[Category: Pommier Y]]

[[Category: Yang W]]

[[Category: Yang X]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9ca0 is ON HOLD
+==Human TOP3B-TDRD3 core complex in DNA pre-cleavage state==
+<StructureSection load='9ca0' size='340' side='right'caption='[[9ca0]], [[Resolution|resolution]] 3.48&Aring;' scene=''>
-Authors: Yang, X., Chen, X., Yang, W., Pommier, Y.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9ca0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CA0 FirstGlance]. <br>
-Description: Human TOP3B-TDRD3 core complex in DNA pre-cleavage state
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.48&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
-[[Category: Chen, X]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ca0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ca0 OCA], [https://pdbe.org/9ca0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ca0 RCSB], [https://www.ebi.ac.uk/pdbsum/9ca0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ca0 ProSAT]</span></td></tr>
-[[Category: Yang, W]]
+</table>
-[[Category: Yang, X]]
+== Function ==
-[[Category: Pommier, Y]]
+[https://www.uniprot.org/uniprot/TOP3B_HUMAN TOP3B_HUMAN] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Chen X]]
+[[Category: Pommier Y]]
+[[Category: Yang W]]
+[[Category: Yang X]]