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[[Image:1ssj.gif|left|200px]]
{{Seed}}
[[Image:1ssj.png|left|200px]]


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{{STRUCTURE_1ssj|  PDB=1ssj  |  SCENE=  }}  
{{STRUCTURE_1ssj|  PDB=1ssj  |  SCENE=  }}  


'''A DNA DUPLEX CONTAINING A CHOLESTEROL ADDUCT (BETA-ANOMER)'''
===A DNA DUPLEX CONTAINING A CHOLESTEROL ADDUCT (BETA-ANOMER)===




==Overview==
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The three-dimensional solution structure of two DNA decamers of sequence d(CCACXGGAAC)-(GTTCCGGTGG) with a modified nucleotide containing a cholesterol derivative (X) in its C1 '(chol)alpha or C1 '(chol)beta diastereoisomer form has been determined by using NMR and restrained molecular dynamics. This DNA derivative is recognized with high efficiency by the UvrB protein, which is part of the bacterial nucleotide excision repair, and the alpha anomer is repaired more efficiently than the beta one. The structures of the two decamers have been determined from accurate distance constraints obtained from a complete relaxation matrix analysis of the NOE intensities and torsion angle constraints derived from J-coupling constants. The structures have been refined with molecular dynamics methods, including explicit solvent and applying the particle mesh Ewald method to properly evaluate the long range electrostatic interactions. These calculations converge to well defined structures whose conformation is intermediate between the A- and B-DNA families as judged by the root mean square deviation but with sugar puckerings and groove shapes corresponding to a distorted B-conformation. Both duplex adducts exhibit intercalation of the cholesterol group from the major groove of the helix and displacement of the guanine base opposite the modified nucleotide. Based on these structures and molecular dynamics calculations, we propose a tentative model for the recognition of damaged DNA substrates by the UvrB protein.
The line below this paragraph, {{ABSTRACT_PUBMED_15047709}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15047709 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15047709}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSJ OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSJ OCA].  


==Reference==
==Reference==
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Modified dna]]
[[Category: Modified dna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:05:29 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:31:15 2008''

Revision as of 08:31, 28 July 2008

File:1ssj.png

Template:STRUCTURE 1ssj

A DNA DUPLEX CONTAINING A CHOLESTEROL ADDUCT (BETA-ANOMER)

Template:ABSTRACT PUBMED 15047709

About this Structure

Full experimental information is available from OCA.

Reference

Effect of bulky lesions on DNA: solution structure of a DNA duplex containing a cholesterol adduct., Gomez-Pinto I, Cubero E, Kalko SG, Monaco V, van der Marel G, van Boom JH, Orozco M, Gonzalez C, J Biol Chem. 2004 Jun 4;279(23):24552-60. Epub 2004 Mar 31. PMID:15047709

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