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| [[Image:1sza.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1sza| PDB=1sza | SCENE= }} | | {{STRUCTURE_1sza| PDB=1sza | SCENE= }} |
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| '''The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model'''
| | ===The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model=== |
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| ==Overview==
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| During transcription, RNA polymerase (Pol) II synthesizes eukaryotic messenger RNA. Transcription is coupled to RNA processing by the carboxy-terminal domain (CTD) of Pol II, which consists of up to 52 repeats of the sequence Tyr 1-Ser 2-Pro 3-Thr 4-Ser 5-Pro 6-Ser 7 (refs 1, 2). After phosphorylation, the CTD binds tightly to a conserved CTD-interacting domain (CID) present in the proteins Pcf11 and Nrd1, which are essential and evolutionarily conserved factors for polyadenylation-dependent and -independent 3'-RNA processing, respectively. Here we describe the structure of a Ser 2-phosphorylated CTD peptide bound to the CID domain of Pcf11. The CTD motif Ser 2-Pro 3-Thr 4-Ser 5 forms a beta-turn that binds to a conserved groove in the CID domain. The Ser 2 phosphate group does not make direct contact with the CID domain, but may be recognized indirectly because it stabilizes the beta-turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta-spiral model of the CTD. The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15241417}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15241417 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15241417}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Phosphoserine]] | | [[Category: Phosphoserine]] |
| [[Category: Rna polymerase ii ctd interacting domain]] | | [[Category: Rna polymerase ii ctd interacting domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:18:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:11:56 2008'' |
