Function
Syntaxin (Stx) are membrane-integrated proteins that are instrumental in exocytosis of vesicles.
- Stx-1 is essential for the formation of the SNARE complex involved in the fusion machinery in the brain. Stx-1 forms a 4-helical bundle complex with proteins SNAP-25 and VAMP2 which drives fusion of vesicles with the plasma membrane[1]. The coiled-coil bundle structure is formed by the interactions of the complex SNARE motifs[2].
- Stx-1A regulates the function of transmembrane proteins like ion channels and neurotransmitter transporters[3].
Relevance
The blockade of Stx-1 inhibits glioblastoma tumor growth and may represent a novel therapeutic tool against this fatal tumor[4].
Structural highlights
Syntaxin 1a SNARE motif complex with VAMP2 SNARE motif and SNAP25 SNARE motif (PDB code 3hd7). The interactions of the syntaxin 1a and VAMP2 SNARE motives consist of hydrogen bond and polar interactions[5]. The interactions of the syntaxin 1a with AMP2 SNARE motif and SNAP25 SNARE motives (PDB code 3ipd).
3D structures of syntaxin
Syntaxin 3D structures
References
- ↑ Hussain S, Ringsevjen H, Egbenya DL, Skjervold TL, Davanger S. SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus. Front Mol Neurosci. 2016 Feb 5;9:10. doi: 10.3389/fnmol.2016.00010. eCollection, 2016. PMID:26903802 doi:https://dx.doi.org/10.3389/fnmol.2016.00010
- ↑ Liang B, Kiessling V, Tamm LK. Prefusion structure of syntaxin-1A suggests pathway for folding into neuronal trans-SNARE complex fusion intermediate. Proc Natl Acad Sci U S A. 2013 Nov 11. PMID:24218570 doi:https://dx.doi.org/10.1073/pnas.1314699110
- ↑ Binda F, Dipace C, Bowton E, Robertson SD, Lute BJ, Fog JU, Zhang M, Sen N, Colbran RJ, Gnegy ME, Gether U, Javitch JA, Erreger K, Galli A. Syntaxin 1A interaction with the dopamine transporter promotes amphetamine-induced dopamine efflux. Mol Pharmacol. 2008 Oct;74(4):1101-8. PMID:18617632 doi:10.1124/mol.108.048447
- ↑ Hussain S, Ringsevjen H, Egbenya DL, Skjervold TL, Davanger S. SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus. Front Mol Neurosci. 2016 Feb 5;9:10. doi: 10.3389/fnmol.2016.00010. eCollection, 2016. PMID:26903802 doi:https://dx.doi.org/10.3389/fnmol.2016.00010
- ↑ Stein A, Weber G, Wahl MC, Jahn R. Helical extension of the neuronal SNARE complex into the membrane. Nature. 2009 Jul 23;460(7254):525-8. Epub 2009 Jul 1. PMID:19571812 doi:10.1038/nature08156
