9j1n: Difference between revisions

Latest revision as of 11:05, 18 June 2025

Mouse Spi-B Ets domain in complex with DNA containing AGAA sequence

Structural highlights

9j1n is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.24Å
Ligands:	3D1, THM
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPIB_MOUSE Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Garrett-Sinha LA, Su GH, Rao S, Kabak S, Hao Z, Clark MR, Simon MC. PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction. Immunity. 1999 Apr;10(4):399-408. PMID:10229183 doi:10.1016/s1074-7613(00)80040-0
↑ Garrett-Sinha LA, Dahl R, Rao S, Barton KP, Simon MC. PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. Blood. 2001 May 1;97(9):2908-12. PMID:11313289 doi:10.1182/blood.v97.9.2908
↑ Hu CJ, Rao S, Ramirez-Bergeron DL, Garrett-Sinha LA, Gerondakis S, Clark MR, Simon MC. PU.1/Spi-B regulation of c-rel is essential for mature B cell survival. Immunity. 2001 Oct;15(4):545-55. PMID:11672537 doi:10.1016/s1074-7613(01)00219-9
↑ Dahl R, Ramirez-Bergeron DL, Rao S, Simon MC. Spi-B can functionally replace PU.1 in myeloid but not lymphoid development. EMBO J. 2002 May 1;21(9):2220-30. PMID:11980719 doi:10.1093/emboj/21.9.2220
↑ Kim N, Martin TE, Simon MC, Storb U. The transcription factor Spi-B is not required for somatic hypermutation. Mol Immunol. 2003 Jan;39(10):577-83. PMID:12431391 doi:10.1016/s0161-5890(02)00201-8
↑ Su GH, Ip HS, Cobb BS, Lu MM, Chen HM, Simon MC. The Ets protein Spi-B is expressed exclusively in B cells and T cells during development. J Exp Med. 1996 Jul 1;184(1):203-14. PMID:8691135 doi:10.1084/jem.184.1.203
↑ Su GH, Chen HM, Muthusamy N, Garrett-Sinha LA, Baunoch D, Tenen DG, Simon MC. Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B. EMBO J. 1997 Dec 1;16(23):7118-29. PMID:9384589 doi:10.1093/emboj/16.23.7118

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Garrett-Sinha LA, Su GH, Rao S, Kabak S, Hao Z, Clark MR, Simon MC. PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction. Immunity. 1999 Apr;10(4):399-408. PMID:10229183 doi:10.1016/s1074-7613(00)80040-0

[2] Garrett-Sinha LA, Dahl R, Rao S, Barton KP, Simon MC. PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. Blood. 2001 May 1;97(9):2908-12. PMID:11313289 doi:10.1182/blood.v97.9.2908

[3] Hu CJ, Rao S, Ramirez-Bergeron DL, Garrett-Sinha LA, Gerondakis S, Clark MR, Simon MC. PU.1/Spi-B regulation of c-rel is essential for mature B cell survival. Immunity. 2001 Oct;15(4):545-55. PMID:11672537 doi:10.1016/s1074-7613(01)00219-9

[4] Dahl R, Ramirez-Bergeron DL, Rao S, Simon MC. Spi-B can functionally replace PU.1 in myeloid but not lymphoid development. EMBO J. 2002 May 1;21(9):2220-30. PMID:11980719 doi:10.1093/emboj/21.9.2220

[5] Kim N, Martin TE, Simon MC, Storb U. The transcription factor Spi-B is not required for somatic hypermutation. Mol Immunol. 2003 Jan;39(10):577-83. PMID:12431391 doi:10.1016/s0161-5890(02)00201-8

[6] Su GH, Ip HS, Cobb BS, Lu MM, Chen HM, Simon MC. The Ets protein Spi-B is expressed exclusively in B cells and T cells during development. J Exp Med. 1996 Jul 1;184(1):203-14. PMID:8691135 doi:10.1084/jem.184.1.203

[7] Su GH, Chen HM, Muthusamy N, Garrett-Sinha LA, Baunoch D, Tenen DG, Simon MC. Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B. EMBO J. 1997 Dec 1;16(23):7118-29. PMID:9384589 doi:10.1093/emboj/16.23.7118

[1]

[2]

[3]

[4]

[5]

[6]

[7]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9j1n is ON HOLD  until Paper Publication
+==Mouse Spi-B Ets domain in complex with DNA containing AGAA sequence==
+<StructureSection load='9j1n' size='340' side='right'caption='[[9j1n]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
-Authors: Nonaka, Y., Kamitori, S.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9j1n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9J1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9J1N FirstGlance]. <br>
-Description: Mouse Spi-B Ets domain in complex with DNA containing AGAA sequence
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3D1:(2R,3S,5R)-5-(6-AMINO-9H-PURIN-9-YL)-TETRAHYDRO-2-(HYDROXYMETHYL)FURAN-3-OL'>3D1</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
-[[Category: Kamitori, S]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9j1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9j1n OCA], [https://pdbe.org/9j1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9j1n RCSB], [https://www.ebi.ac.uk/pdbsum/9j1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9j1n ProSAT]</span></td></tr>
-[[Category: Nonaka, Y]]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPIB_MOUSE SPIB_MOUSE] Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.<ref>PMID:10229183</ref> <ref>PMID:11313289</ref> <ref>PMID:11672537</ref> <ref>PMID:11980719</ref> <ref>PMID:12431391</ref> <ref>PMID:8691135</ref> <ref>PMID:9384589</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Kamitori S]]
+[[Category: Nonaka Y]]

9j1n: Difference between revisions

Latest revision as of 11:05, 18 June 2025

Mouse Spi-B Ets domain in complex with DNA containing AGAA sequence

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools