1t7a: Difference between revisions

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[[Image:1t7a.jpg|left|200px]]
{{Seed}}
[[Image:1t7a.png|left|200px]]


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{{STRUCTURE_1t7a|  PDB=1t7a  |  SCENE=  }}  
{{STRUCTURE_1t7a|  PDB=1t7a  |  SCENE=  }}  


'''Crystal structure of mutant Lys8Asp of scorpion alpha-like neurotoxin BmK M1 from Buthus martensii Karsch'''
===Crystal structure of mutant Lys8Asp of scorpion alpha-like neurotoxin BmK M1 from Buthus martensii Karsch===




==Overview==
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Non-proline cis peptide bonds have been observed in numerous protein crystal structures even though the energetic barrier to this conformation is significant and no non-prolyl-cis/trans-isomerase has been identified to date. While some external factors, such as metal binding or co-factor interaction, have been identified that appear to induce cis/trans isomerization of non-proline peptide bonds, the intrinsic structural basis for their existence and the mechanism governing cis/trans isomerization in proteins remains poorly understood. Here, we report the crystal structure of a newly isolated neurotoxin, the scorpion alpha-like toxin Buthus martensii Karsch (BmK) M7, at 1.4A resolution. BmK M7 crystallizes as a dimer in which the identical non-proline peptide bond between residues 9 and 10 exists either in the cis conformation or as a mixture of cis and trans conformations in either monomer. We also determined the crystal structures of several mutants of BmK M1, a representative scorpion alpha-like toxin that contains an identical non-proline cis peptide bond as that observed in BmK M7, in which residues within or neighboring the cis peptide bond were altered. Substitution of an aspartic acid residue for lysine at residue 8 in the BmK M1 (K8D) mutant converted the cis form of the non-proline peptide bond 9-10 into the trans form, revealing an intramolecular switch for cis-to-trans isomerization. Cis/trans interconversion of the switch residue at position 8 appears to be sequence-dependent as the peptide bond between residues 9 and 10 retains its wild-type cis conformation in the BmK M1 (K8Q) mutant structure. The structural interconversion of the isomeric states of the BmK M1 non-proline cis peptide bond may relate to the conversion of the scorpion alpha-toxins subgroups.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural mechanism governing cis and trans isomeric states and an intramolecular switch for cis/trans isomerization of a non-proline peptide bond observed in crystal structures of scorpion toxins., Guan RJ, Xiang Y, He XL, Wang CG, Wang M, Zhang Y, Sundberg EJ, Wang DC, J Mol Biol. 2004 Aug 27;341(5):1189-204. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15321715 15321715]
Structural mechanism governing cis and trans isomeric states and an intramolecular switch for cis/trans isomerization of a non-proline peptide bond observed in crystal structures of scorpion toxins., Guan RJ, Xiang Y, He XL, Wang CG, Wang M, Zhang Y, Sundberg EJ, Wang DC, J Mol Biol. 2004 Aug 27;341(5):1189-204. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15321715 15321715]
Crystal structures of two alpha-like scorpion toxins: non-proline cis peptide bonds and implications for new binding site selectivity on the sodium channel., He XL, Li HM, Zeng ZH, Liu XQ, Wang M, Wang DC, J Mol Biol. 1999 Sep 10;292(1):125-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10493862 10493862]
Expression and purification of the BmK M1 neurotoxin from the scorpion Buthus martensii Karsch., Shao F, Xiong YM, Zhu RH, Ling MH, Chi CW, Wang DC, Protein Expr Purif. 1999 Dec;17(3):358-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600453 10600453]
The CDNA and genomic DNA sequences of a mammalian neurotoxin from the scorpion Buthus martensii Karsch., Xiong YM, Ling MH, Wang DC, Chi CW, Toxicon. 1997 Jul;35(7):1025-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9248001 9248001]
[[Category: Mesobuthus martensii]]
[[Category: Mesobuthus martensii]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Non-proline peptide bond]]
[[Category: Non-proline peptide bond]]
[[Category: Scorpion toxin]]
[[Category: Scorpion toxin]]
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