1t7d: Difference between revisions

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[[Image:1t7d.gif|left|200px]]
{{Seed}}
[[Image:1t7d.png|left|200px]]


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{{STRUCTURE_1t7d|  PDB=1t7d  |  SCENE=  }}  
{{STRUCTURE_1t7d|  PDB=1t7d  |  SCENE=  }}  


'''Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor'''
===Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor===




==Overview==
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We report here the crystallographic and biophysical analysis of a soluble, catalytically active fragment of the Escherichia coli type I signal peptidase (SPase Delta2-75) in complex with arylomycin A2. The 2.5-A resolution structure revealed that the inhibitor is positioned with its COOH-terminal carboxylate oxygen (O45) within hydrogen bonding distance of all the functional groups in the catalytic center of the enzyme (Ser90 O-gamma, Lys145 N-zeta, and Ser88 O-gamma) and that it makes beta-sheet type interactions with the beta-strands that line each side of the binding site. Ligand binding studies, calorimetry, fluorescence spectroscopy, and stopped-flow kinetics were also used to analyze the binding mode of this unique non-covalently bound inhibitor. The crystal structure was solved in the space group P4(3)2(1)2. A detailed comparison is made to the previously published acyl-enzyme inhibitor complex structure (space group: P2(1)2(1)2) and the apo-enzyme structure (space group: P4(1)2(1)2). Together this work provides insights into the binding of pre-protein substrates to signal peptidase and will prove helpful in the development of novel antibiotics.
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{{ABSTRACT_PUBMED_15136583}}


==About this Structure==
==About this Structure==
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[[Category: Signal peptidase]]
[[Category: Signal peptidase]]
[[Category: Signal peptide]]
[[Category: Signal peptide]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:37:36 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:14:45 2008''

Revision as of 13:14, 27 July 2008

File:1t7d.png

Template:STRUCTURE 1t7d

Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor

Template:ABSTRACT PUBMED 15136583

About this Structure

1T7D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor., Paetzel M, Goodall JJ, Kania M, Dalbey RE, Page MG, J Biol Chem. 2004 Jul 16;279(29):30781-90. Epub 2004 May 10. PMID:15136583

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