|
|
| Line 1: |
Line 1: |
| [[Image:1t7x.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1t7x.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1t7x| PDB=1t7x | SCENE= }} | | {{STRUCTURE_1t7x| PDB=1t7x | SCENE= }} |
|
| |
|
| '''Zn-alpha-2-glycoprotein; refolded CHO-ZAG PEG 400'''
| | ===Zn-alpha-2-glycoprotein; refolded CHO-ZAG PEG 400=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex (MHC) molecules and revealed a non-peptidic ligand in the ZAG counterpart of the MHC peptide-binding groove. Here we present crystallographic studies to explore further the nature of the non-peptidic ligand in the ZAG groove. Comparison of the structures of several forms of recombinant ZAG, including a 1.95 A structure derived from ZAG expressed in insect cells, suggests that the non-peptidic ligand in the current structures and in the structure of serum ZAG is a polyethylene glycol (PEG), which is present in the crystallization conditions used. Further support for PEG binding in the ZAG groove is provided by the finding that PEG displaces a fluorophore-tagged fatty acid from the ZAG binding site. From these results we hypothesize that our purified forms of ZAG do not contain a bound endogenous ligand, but that the ZAG groove is capable of binding hydrophobic molecules, which may relate to its function.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15477100}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15477100 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15477100}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 28: |
Line 32: |
| [[Category: McDermott, L.]] | | [[Category: McDermott, L.]] |
| [[Category: Mhc class i homolog]] | | [[Category: Mhc class i homolog]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:38:53 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:59:40 2008'' |
