1th1: Difference between revisions

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[[Image:1th1.~~gif~~|left|200px]]

[[Image:1th1.png|left|200px]]

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~~'''~~Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment~~'''~~

===Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment===

~~==Overview==~~

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The ~~tumor suppressor adenomatous polyposis coli (APC) plays a critical role in the turnover of cytosolic beta-catenin~~, the ~~key effector of~~ the ~~canonical Wnt signaling pathway. APC contains seven 20 amino acid~~ (~~20 aa) beta-catenin binding repeats that are required for beta-catenin turnover~~. ~~We have determined the crystal structure of beta-catenin in complex with a phosphorylated APC fragment containing two 20 aa repeats~~. ~~Surprisingly, one single phosphorylated 20 aa repeat~~, ~~together with its flanking regions, covers~~ the ~~entire structural groove of beta-catenin and may thus compete for beta-catenin binding with all other beta-catenin armadillo repeat partners~~. ~~Our biochemical studies show that phosphorylation of the APC 20 aa repeats increases the affinity of the repeats for beta~~-~~catenin by 300~~- ~~to 500-fold and the phosphorylated 20 aa repeats prevent beta-catenin binding to Tcf. Our work suggests that the phosphorylation of the APC 20 aa repeats could be a critical switch for APC function.~~

The line below this paragraph, {{ABSTRACT_PUBMED_15327769}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15327769 is the PubMed ID number.

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==About this Structure==

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[[Category: Xu, W.]]

[[Category: Protein-protein complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 09~~:56:19 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:11:02 2008''

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-[[Image:1th1.gif|left|200px]]
+{{Seed}}
+[[Image:1th1.png|left|200px]]
 <!--
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 {{STRUCTURE_1th1|  PDB=1th1  |  SCENE=  }}
-'''Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment'''
+===Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment===
-==Overview==
+<!--
-The tumor suppressor adenomatous polyposis coli (APC) plays a critical role in the turnover of cytosolic beta-catenin, the key effector of the canonical Wnt signaling pathway. APC contains seven 20 amino acid (20 aa) beta-catenin binding repeats that are required for beta-catenin turnover. We have determined the crystal structure of beta-catenin in complex with a phosphorylated APC fragment containing two 20 aa repeats. Surprisingly, one single phosphorylated 20 aa repeat, together with its flanking regions, covers the entire structural groove of beta-catenin and may thus compete for beta-catenin binding with all other beta-catenin armadillo repeat partners. Our biochemical studies show that phosphorylation of the APC 20 aa repeats increases the affinity of the repeats for beta-catenin by 300- to 500-fold and the phosphorylated 20 aa repeats prevent beta-catenin binding to Tcf. Our work suggests that the phosphorylation of the APC 20 aa repeats could be a critical switch for APC function.
+The line below this paragraph, {{ABSTRACT_PUBMED_15327769}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15327769 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15327769}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Xu, W.]]
 [[Category: Protein-protein complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:56:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:11:02 2008''