1tkq: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:1tkq.gif|left|200px]]
{{Seed}}
[[Image:1tkq.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1tkq|  PDB=1tkq  |  SCENE=  }}  
{{STRUCTURE_1tkq|  PDB=1tkq  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsCl'''
===SOLUTION STRUCTURE OF A LINKED UNSYMMETRIC GRAMICIDIN IN A MEMBRANE-ISOELECTRICAL SOLVENTS MIXTURE IN THE PRESENCE OF CsCl===




==Overview==
<!--  
The biological ion channel gramicidin A (gA) was modified by synthetic means to obtain the tail-to-tail linked asymmetric gA-derived dimer compound 3. Single-channel current measurements for 3 in planar lipid bilayers exhibit an Eisenman I ion selectivity for alkali cations. The structural asymmetry does not lead to an observable functional asymmetry. The structure of 3 in solution without and with Cs cations was investigated by 1H-NMR spectroscopy. In CDCl3/CD3OH (1 : 1, v/v), 3 forms a mixture of double-stranded beta-helices. Upon addition of excess CsCl, the double-stranded species are converted completely into one new conformer: the right-handed single-stranded beta-helix. A combination of DQF-COSY and TOCSY was used for the assignment of the 1H-NMR spectrum of the Cs-3 complex in CDCl3/CD3OH (1 : 1, v/v). A total of 69 backbone, 27 long-range, and 64 side-chain distance restraints were obtained from NOESY together with 25 phi and 14 chi1 torsion angles obtained from coupling constants. These data were used as input for structure calculation with dyana built in sybyl 6.8. A final set of 11 structures with an average rmsd for the backbone of 0.45 A was obtained (PDB: 1TKQ). The structure of the Cs-3 complex in solution is equivalent to the bioactive channel conformation in the membrane environment.
The line below this paragraph, {{ABSTRACT_PUBMED_15691331}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15691331 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_15691331}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKQ OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKQ OCA].  


==Reference==
==Reference==
An asymmetric ion channel derived from gramicidin A. Synthesis, function and NMR structure., Xie X, Al-Momani L, Reiss P, Griesinger C, Koert U, FEBS J. 2005 Feb;272(4):975-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15691331 15691331]
An asymmetric ion channel derived from gramicidin A. Synthesis, function and NMR structure., Xie X, Al-Momani L, Reiss P, Griesinger C, Koert U, FEBS J. 2005 Feb;272(4):975-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15691331 15691331]
High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR., Ketchem RR, Hu W, Cross TA, Science. 1993 Sep 10;261(5127):1457-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7690158 7690158]
Solution structure of a parallel left-handed double-helical gramicidin-A determined by 2D 1H NMR., Chen Y, Tucker A, Wallace BA, J Mol Biol. 1996 Dec 13;264(4):757-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8980684 8980684]
Cation control in functional helical programming: structures of a D,L-peptide ion channel., Arndt HD, Bockelmann D, Knoll A, Lamberth S, Griesinger C, Koert U, Angew Chem Int Ed Engl. 2002 Nov 4;41(21):4062-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12412082 12412082]
[[Category: Al-Momani, L.]]
[[Category: Al-Momani, L.]]
[[Category: Bockelmann, D.]]
[[Category: Bockelmann, D.]]
Line 26: Line 36:
[[Category: Xie, X.]]
[[Category: Xie, X.]]
[[Category: Beta-helix]]
[[Category: Beta-helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:04:11 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:54:01 2008''
Retrieved from "https://proteopedia.org/1tkq"