|
|
| Line 1: |
Line 1: |
| [[Image:1ts8.gif|left|200px]] | | {{Seed}} |
| | [[Image:1ts8.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1ts8| PDB=1ts8 | SCENE= }} | | {{STRUCTURE_1ts8| PDB=1ts8 | SCENE= }} |
|
| |
|
| '''Structure of the pR cis planar intermediate from time-resolved Laue crystallography'''
| | ===Structure of the pR cis planar intermediate from time-resolved Laue crystallography=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved Laue crystallography to extract short-lived intermediate structures and thereby unveil signal transduction in the blue light photoreceptor photoactive yellow protein (PYP) from Halorhodospira halophila. By analyzing a comprehensive set of Laue data during the PYP photocycle (forty-seven time points from one nanosecond to one second), we track all atoms in PYP during its photocycle and directly observe how absorption of a blue light photon by its p-coumaric acid chromophore triggers a reversible photocycle. We identify a complex chemical mechanism characterized by five distinct structural intermediates. Structural changes at the chromophore in the early, red-shifted intermediates are transduced to the exterior of the protein in the late, blue-shifted intermediates through an initial "volume-conserving" isomerization of the chromophore and the progressive disruption of hydrogen bonds between the chromophore and its surrounding binding pocket. These results yield a comprehensive view of the PYP photocycle when seen in the light of previous biophysical studies on the system.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15870207}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15870207 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15870207}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 33: |
Line 37: |
| [[Category: Wulff, M.]] | | [[Category: Wulff, M.]] |
| [[Category: Photoreceptor]] | | [[Category: Photoreceptor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:18:29 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 12:39:00 2008'' |
