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[[Image:1tte.gif|left|200px]]
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[[Image:1tte.png|left|200px]]


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{{STRUCTURE_1tte|  PDB=1tte  |  SCENE=  }}  
{{STRUCTURE_1tte|  PDB=1tte  |  SCENE=  }}  


'''The Structure of a Class II ubiquitin-conjugating enzyme, Ubc1.'''
===The Structure of a Class II ubiquitin-conjugating enzyme, Ubc1.===




==Overview==
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E2 conjugating enzymes form a thiol ester intermediate with ubiquitin, which is subsequently transferred to a substrate protein targeted for degradation. While all E2 proteins comprise a catalytic domain where the thiol ester is formed, several E2s (class II) have C-terminal extensions proposed to control substrate recognition, dimerization, or polyubiquitin chain formation. Here we present the novel solution structure of the class II E2 conjugating enzyme Ubc1 from Saccharomyces cerevisiae. The structure shows the N-terminal catalytic domain adopts an alpha/beta fold typical of other E2 proteins. This domain is physically separated from its C-terminal domain by a 22-residue flexible tether. The C-terminal domain adopts a three-helix bundle that we have identified as an ubiquitin-associated domain (UBA). NMR chemical shift perturbation experiments show this UBA domain interacts in a regioselective manner with ubiquitin. This two-domain structure of Ubc1 was used to identify other UBA-containing class II E2 proteins, including human E2-25K, that likely have a similar architecture and to determine the role of the UBA domain in facilitating polyubiquitin chain formation.
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{{ABSTRACT_PUBMED_15328341}}


==About this Structure==
==About this Structure==
1TTE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTE OCA].  
1TTE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTE OCA].  


==Reference==
==Reference==
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[[Category: Ubc1]]
[[Category: Ubc1]]
[[Category: Ubiquitin-dependent degradation]]
[[Category: Ubiquitin-dependent degradation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:20:30 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:04:21 2008''

Revision as of 09:04, 29 July 2008

File:1tte.png

Template:STRUCTURE 1tte

The Structure of a Class II ubiquitin-conjugating enzyme, Ubc1.

Template:ABSTRACT PUBMED 15328341

About this Structure

1TTE is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Solution structure of the flexible class II ubiquitin-conjugating enzyme Ubc1 provides insights for polyubiquitin chain assembly., Merkley N, Shaw GS, J Biol Chem. 2004 Nov 5;279(45):47139-47. Epub 2004 Aug 24. PMID:15328341

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