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| [[Image:1tu7.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1tu7| PDB=1tu7 | SCENE= }} | | {{STRUCTURE_1tu7| PDB=1tu7 | SCENE= }} |
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| '''Structure of Onchocerca Volvulus Pi-class Glutathione S-transferase'''
| | ===Structure of Onchocerca Volvulus Pi-class Glutathione S-transferase=== |
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| ==Overview==
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| Onchocerciasis is a debilitating parasitic disease caused by the filarial worm Onchocerca volvulus. Similar to other helminth parasites, O. volvulus is capable of evading the host's immune responses by a variety of defense mechanisms, including the detoxification activities of the glutathione S-transferases (GSTs). Additionally, in response to drug treatment, helminth GSTs are highly up-regulated, making them tempting targets both for chemotherapy and for vaccine development. We analyzed the three-dimensional x-ray structure of the major cytosolic GST from O. volvulus (Ov-GST2) in complex with its natural substrate glutathione and its competitive inhibitor S-hexylglutathione at 1.5 and 1.8 angstrom resolution, respectively. From the perspective of the biochemical classification, the Ov-GST2 seems to be related to pi-class GSTs. However, in comparison to other pi-class GSTs, in particular to the host's counterpart, the Ov-GST2 reveals significant and unusual differences in the sequence and overall structure. Major differences can be found in helix alpha-2, an important region for substrate recognition. Moreover, the binding site for the electrophilic co-substrate is spatially increased and more solvent-accessible. These structural alterations are responsible for different substrate specificities and will form the basis of parasite-specific structure-based drug design investigations.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15640152}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15640152 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15640152}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Perbandt, M.]] | | [[Category: Perbandt, M.]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:22:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:22:33 2008'' |
