9gtv: Difference between revisions

Latest revision as of 09:33, 25 December 2024

Crystal structure of RamR with Tyr59 replaced with para-boronophenylalanine (boronate form)

Structural highlights

9gtv is a 4 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.78Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HTHTR_SALTY Transcriptional regulator (PubMed:18443112, PubMed:22948865, PubMed:23800819, PubMed:30655545). Represses the transcription of the transcriptional activator RamA and, thereby, leads to repression of the expression of the efflux pump subunits AcrA and AcrB, and TolC (PubMed:18443112, PubMed:22948865, PubMed:23800819, PubMed:30655545). Acts by binding directly to the promoter region of the ramA gene (PubMed:22123696, PubMed:22948865, PubMed:23800819). Promoter binding may be inhibited partially by the small regulatory RNA StyR3, perhaps thereby ensuring a basal level of expression of RamA.[UniProtKB:A0A0H3GJQ8]^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Abouzeed YM, Baucheron S, Cloeckaert A. ramR mutations involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother. 2008 Jul;52(7):2428-34. PMID:18443112 doi:10.1128/AAC.00084-08
↑ Baucheron S, Coste F, Canepa S, Maurel MC, Giraud E, Culard F, Castaing B, Roussel A, Cloeckaert A. Binding of the RamR repressor to wild-type and mutated promoters of the RamA gene involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother. 2012 Feb;56(2):942-8. PMID:22123696 doi:10.1128/AAC.05444-11
↑ Ricci V, Busby SJ, Piddock LJ. Regulation of RamA by RamR in Salmonella enterica serovar Typhimurium: isolation of a RamR superrepressor. Antimicrob Agents Chemother. 2012 Nov;56(11):6037-40. PMID:22948865 doi:10.1128/AAC.01320-12
↑ Yamasaki S, Nikaido E, Nakashima R, Sakurai K, Fujiwara D, Fujii I, Nishino K. The crystal structure of multidrug-resistance regulator RamR with multiple drugs. Nat Commun. 2013 Jun 26;4:2078. doi: 10.1038/ncomms3078. PMID:23800819 doi:10.1038/ncomms3078
↑ Yamasaki S, Nakashima R, Sakurai K, Baucheron S, Giraud E, Doublet B, Cloeckaert A, Nishino K. Crystal structure of the multidrug resistance regulator RamR complexed with bile acids. Sci Rep. 2019 Jan 17;9(1):177. doi: 10.1038/s41598-018-36025-8. PMID:30655545 doi:https://dx.doi.org/10.1038/s41598-018-36025-8

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Abouzeed YM, Baucheron S, Cloeckaert A. ramR mutations involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother. 2008 Jul;52(7):2428-34. PMID:18443112 doi:10.1128/AAC.00084-08

[2] Baucheron S, Coste F, Canepa S, Maurel MC, Giraud E, Culard F, Castaing B, Roussel A, Cloeckaert A. Binding of the RamR repressor to wild-type and mutated promoters of the RamA gene involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother. 2012 Feb;56(2):942-8. PMID:22123696 doi:10.1128/AAC.05444-11

[3] Ricci V, Busby SJ, Piddock LJ. Regulation of RamA by RamR in Salmonella enterica serovar Typhimurium: isolation of a RamR superrepressor. Antimicrob Agents Chemother. 2012 Nov;56(11):6037-40. PMID:22948865 doi:10.1128/AAC.01320-12

[4] Yamasaki S, Nikaido E, Nakashima R, Sakurai K, Fujiwara D, Fujii I, Nishino K. The crystal structure of multidrug-resistance regulator RamR with multiple drugs. Nat Commun. 2013 Jun 26;4:2078. doi: 10.1038/ncomms3078. PMID:23800819 doi:10.1038/ncomms3078

[5] Yamasaki S, Nakashima R, Sakurai K, Baucheron S, Giraud E, Doublet B, Cloeckaert A, Nishino K. Crystal structure of the multidrug resistance regulator RamR complexed with bile acids. Sci Rep. 2019 Jan 17;9(1):177. doi: 10.1038/s41598-018-36025-8. PMID:30655545 doi:https://dx.doi.org/10.1038/s41598-018-36025-8

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9gtv is ON HOLD  until Paper Publication
+==Crystal structure of RamR with Tyr59 replaced with para-boronophenylalanine (boronate form)==
+<StructureSection load='9gtv' size='340' side='right'caption='[[9gtv]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
-Authors: Longwitz, L., Brouwer, B., Thunnissen, A.M.W.H., Roelfes, G.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9gtv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GTV FirstGlance]. <br>
-Description: Crystal structure of RamR with Tyr59 replaced with para-boronophenylalanine (boronate form)
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.78&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gtv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gtv OCA], [https://pdbe.org/9gtv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gtv RCSB], [https://www.ebi.ac.uk/pdbsum/9gtv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gtv ProSAT]</span></td></tr>
-[[Category: Thunnissen, A.M.W.H]]
+</table>
-[[Category: Brouwer, B]]
+== Function ==
-[[Category: Longwitz, L]]
+[https://www.uniprot.org/uniprot/HTHTR_SALTY HTHTR_SALTY] Transcriptional regulator (PubMed:18443112, PubMed:22948865, PubMed:23800819, PubMed:30655545). Represses the transcription of the transcriptional activator RamA and, thereby, leads to repression of the expression of the efflux pump subunits AcrA and AcrB, and TolC (PubMed:18443112, PubMed:22948865, PubMed:23800819, PubMed:30655545). Acts by binding directly to the promoter region of the ramA gene (PubMed:22123696, PubMed:22948865, PubMed:23800819). Promoter binding may be inhibited partially by the small regulatory RNA StyR3, perhaps thereby ensuring a basal level of expression of RamA.[UniProtKB:A0A0H3GJQ8]<ref>PMID:18443112</ref> <ref>PMID:22123696</ref> <ref>PMID:22948865</ref> <ref>PMID:23800819</ref> <ref>PMID:30655545</ref>
-[[Category: Roelfes, G]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
+[[Category: Brouwer B]]
+[[Category: Longwitz L]]
+[[Category: Roelfes G]]
+[[Category: Thunnissen AMWH]]

9gtv: Difference between revisions

Latest revision as of 09:33, 25 December 2024

Crystal structure of RamR with Tyr59 replaced with para-boronophenylalanine (boronate form)

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools