1u0i: Difference between revisions

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[[Image:1u0i.jpg|left|200px]]
{{Seed}}
[[Image:1u0i.png|left|200px]]


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{{STRUCTURE_1u0i|  PDB=1u0i  |  SCENE=  }}  
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'''IAAL-E3/K3 heterodimer'''
===IAAL-E3/K3 heterodimer===




==Overview==
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The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design.
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{{ABSTRACT_PUBMED_15457434}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA].  


==Reference==
==Reference==
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[[Category: Sykes, B D.]]
[[Category: Sykes, B D.]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:36:00 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:38:23 2008''

Revision as of 13:38, 28 July 2008

File:1u0i.png

Template:STRUCTURE 1u0i

IAAL-E3/K3 heterodimer

Template:ABSTRACT PUBMED 15457434

About this Structure

Full experimental information is available from OCA.

Reference

NMR solution structure of a highly stable de novo heterodimeric coiled-coil., Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD, Biopolymers. 2004 Dec 5;75(5):367-75. PMID:15457434

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