1u0v: Difference between revisions

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[[Image:1u0v.gif|left|200px]]
{{Seed}}
[[Image:1u0v.png|left|200px]]


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{{STRUCTURE_1u0v|  PDB=1u0v  |  SCENE=  }}  
{{STRUCTURE_1u0v|  PDB=1u0v  |  SCENE=  }}  


'''An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization of Specificity of Type III Polyketide Synthases: 18xCHS structure'''
===An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization of Specificity of Type III Polyketide Synthases: 18xCHS structure===




==Overview==
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Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
The line below this paragraph, {{ABSTRACT_PUBMED_15380179}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_15380179}}


==About this Structure==
==About this Structure==
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[[Category: Thiolase fold]]
[[Category: Thiolase fold]]
[[Category: Type iii polyketide synthase]]
[[Category: Type iii polyketide synthase]]
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