1u19: Difference between revisions

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[[Image:1u19.gif|left|200px]]
{{Seed}}
[[Image:1u19.png|left|200px]]


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{{STRUCTURE_1u19|  PDB=1u19  |  SCENE=  }}  
{{STRUCTURE_1u19|  PDB=1u19  |  SCENE=  }}  


'''Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution'''
===Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution===




==Overview==
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A new high-resolution structure is reported for bovine rhodopsin, the visual pigment in rod photoreceptor cells. Substantial improvement of the resolution limit to 2.2 A has been achieved by new crystallization conditions, which also reduce significantly the probability of merohedral twinning in the crystals. The new structure completely resolves the polypeptide chain and provides further details of the chromophore binding site including the configuration about the C6-C7 single bond of the 11-cis-retinal Schiff base. Based on both an earlier structure and the new improved model of the protein, a theoretical study of the chromophore geometry has been carried out using combined quantum mechanics/force field molecular dynamics. The consistency between the experimental and calculated chromophore structures is found to be significantly improved for the 2.2 A model, including the angle of the negatively twisted 6-s-cis-bond. Importantly, the new crystal structure refinement reveals significant negative pre-twist of the C11-C12 double bond and this is also supported by the theoretical calculation although the latter converges to a smaller value. Bond alternation along the unsaturated chain is significant, but weaker in the calculated structure than the one obtained from the X-ray data. Other differences between the experimental and theoretical structures in the chromophore binding site are discussed with respect to the unique spectral properties and excited state reactivity of the chromophore.
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{{ABSTRACT_PUBMED_15327956}}


==About this Structure==
==About this Structure==
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[[Category: Photoreceptor]]
[[Category: Photoreceptor]]
[[Category: Retinal protein]]
[[Category: Retinal protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:37:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:46:24 2008''

Revision as of 04:46, 29 July 2008

File:1u19.png


Drag the structure with the mouse to rotate
1u19, resolution 2.20Å (default scene)
Ligands: BMA, HG, HTG, HTO, PLM, RET, ZN, MAN, NAG
Non-Standard Residues: ACE
Related: 1f88, 1hzx, 1l9h
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Bovine Rhodopsin at 2.2 Angstroms Resolution

Template:ABSTRACT PUBMED 15327956

About this Structure

1U19 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure., Okada T, Sugihara M, Bondar AN, Elstner M, Entel P, Buss V, J Mol Biol. 2004 Sep 10;342(2):571-83. PMID:15327956

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